Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,280,706 |
A→G |
pseudogene (6/402 nt) |
agaW → |
N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,280,706 | 0 | A | G | 100.0%
| 141.4
/ NA
| 41 | pseudogene (6/402 nt) | agaW | N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/22); total (19/22) |
GAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCA > NC_000913/3280631‑3280783
|
gAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAg < 1:614211/81‑1 (MQ=255)
aaTGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGc > 2:614284/1‑81 (MQ=255)
aaTGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGc < 2:614212/81‑1 (MQ=255)
gTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGc < 1:614213/80‑1 (MQ=255)
cAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAgg > 2:614264/1‑80 (MQ=255)
aGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGc < 1:614214/80‑1 (MQ=255)
gggCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCAt < 1:614215/81‑1 (MQ=255)
gCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCAttt < 1:614216/81‑1 (MQ=255)
cGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTg < 1:614218/81‑1 (MQ=255)
cGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTg < 2:614217/81‑1 (MQ=255)
gTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGc > 2:614255/1‑81 (MQ=255)
ccGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGt < 2:614219/80‑1 (MQ=255)
cGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGAGTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTg < 2:614220/81‑1 (MQ=255)
aCAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTggg > 1:614262/1‑81 (MQ=255)
gagCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCAt > 2:614253/1‑81 (MQ=255)
gagCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCAt < 2:614222/81‑1 (MQ=255)
gagCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCAt < 1:614221/81‑1 (MQ=255)
gctgTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGc > 1:614274/1‑81 (MQ=255)
gctgTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGc > 2:614269/1‑81 (MQ=255)
gtgGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCtt > 2:614268/1‑81 (MQ=255)
gtgGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCtt > 2:614272/1‑81 (MQ=255)
tgGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCtt < 1:614223/80‑1 (MQ=255)
tgGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCtt > 1:614261/1‑80 (MQ=255)
ggACCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTa > 1:614263/1‑81 (MQ=255)
aCCTCTTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATc > 2:614259/1‑81 (MQ=255)
tctTTAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCt > 1:614279/1‑81 (MQ=255)
tttAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCt < 1:614224/79‑1 (MQ=255)
tAAATTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTg < 1:614225/78‑1 (MQ=255)
aaaTTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGcc < 2:614226/80‑1 (MQ=255)
aaTTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTg < 2:614227/81‑1 (MQ=255)
aTTACTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTgg < 1:614228/81‑1 (MQ=255)
aCTTTGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGata > 1:614291/1‑81 (MQ=255)
tGAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGtt > 1:614273/1‑81 (MQ=255)
gAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGtt > 2:614238/1‑80 (MQ=255)
gAGGGATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGtt < 1:614229/80‑1 (MQ=255)
gggATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTaa < 1:614230/81‑1 (MQ=255)
ggATTCATCATGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAAc < 2:614231/81‑1 (MQ=255)
tcatcaTGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGcc > 2:614276/1‑81 (MQ=255)
atcatGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTa < 2:614232/81‑1 (MQ=255)
catGGAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAAc > 2:614258/1‑81 (MQ=255)
ggAGATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCa > 2:614285/1‑81 (MQ=255)
|
GAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGAGGGATTCATCATGGAAATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTAACGGCCTAACCCA > NC_000913/3280631‑3280783
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
