Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,400,225 |
T→C |
E288G (GAG→GGG) |
mreB ← |
dynamic cytoskeletal protein MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,400,225 | 0 | T | C | 97.2%
| 116.6
/ ‑4.2
| 36 | E288G (GAG→GGG) | mreB | dynamic cytoskeletal protein MreB |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (16/19); total (17/19) |
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
GAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGG > NC_000913/3400148‑3400301
|
gAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCgg < 2:644279/81‑1 (MQ=255)
gAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCgg < 2:644277/81‑1 (MQ=255)
gAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCg > 2:644333/1‑80 (MQ=255)
aTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGgag < 2:644281/81‑1 (MQ=255)
aTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGgag < 2:644280/81‑1 (MQ=255)
ggTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTc < 1:644282/81‑1 (MQ=255)
tttCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCgg < 2:644283/81‑1 (MQ=255)
cttcCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAg > 2:644334/1‑81 (MQ=255)
ccATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGcc < 1:644284/80‑1 (MQ=255)
cATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTTGGAAGCCAg < 1:644285/81‑1 (MQ=255)
ttAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGtt < 2:644286/81‑1 (MQ=255)
aaCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTcc > 2:644332/1‑81 (MQ=255)
aaCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCcggcg > 2:644348/1‑81 (MQ=255)
cGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCggg < 2:644287/81‑1 (MQ=255)
gTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCa > 1:644352/1‑81 (MQ=255)
aaGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACt < 2:807660/80‑1 (MQ=255)
ggTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGtt > 1:644335/1‑81 (MQ=255)
gTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTc > 1:644337/1‑81 (MQ=255)
gTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTc < 1:644288/81‑1 (MQ=255)
ttACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTc > 2:644356/1‑80 (MQ=255)
cagcagTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGc < 1:644289/81‑1 (MQ=255)
gcagTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCaa < 1:644290/81‑1 (MQ=255)
gcagTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCaa > 2:644359/1‑81 (MQ=255)
gTGCGCCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCa > 1:644338/1‑81 (MQ=255)
gcgcCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAt > 1:644341/1‑80 (MQ=255)
cgcCACCACCGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAtt < 2:644291/80‑1 (MQ=255)
accaccGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACcgc < 1:644292/81‑1 (MQ=255)
caccGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCgcgc > 1:644355/1‑81 (MQ=255)
ccGGTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTc < 2:644293/81‑1 (MQ=255)
ggTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTca > 2:644343/1‑80 (MQ=255)
gTGAGCACCATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTcac > 1:644364/1‑80 (MQ=255)
cATGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGt > 1:644336/1‑81 (MQ=255)
aTGCCGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTc < 2:644294/81‑1 (MQ=255)
ccGCGCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAg < 1:644295/80‑1 (MQ=255)
cgcgCCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCg > 1:644340/1‑81 (MQ=255)
gcgcCCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCgg > 1:644351/1‑81 (MQ=255)
|
GAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGG > NC_000913/3400148‑3400301
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
