Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,402,941 |
A→G |
F131S (TTC→TCC) |
csrD ← |
regulator of CsrB and CsrC decay |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,402,941 | 0 | A | G | 100.0%
| 140.6
/ NA
| 40 | F131S (TTC→TCC) | csrD | regulator of CsrB and CsrC decay |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/21); total (19/21) |
CCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGAAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTC > NC_000913/3402870‑3403017
|
ccGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGc < 1:580721/81‑1 (MQ=255)
cGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGcc > 1:580757/1‑81 (MQ=255)
ggAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCg < 2:580721/81‑1 (MQ=255)
agagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGAt < 1:580724/81‑1 (MQ=255)
agagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGAt < 2:580722/81‑1 (MQ=255)
gagCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATc < 1:580725/81‑1 (MQ=255)
cATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTg < 1:580726/81‑1 (MQ=255)
aCAATGATAAAGCCTATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATa < 2:580726/80‑1 (MQ=255)
aaTGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAAc > 2:580764/1‑81 (MQ=255)
aaTGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAAc < 1:580728/81‑1 (MQ=255)
aaGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACg < 2:580728/80‑1 (MQ=255)
cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAAc > 1:580782/1‑80 (MQ=255)
cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACg < 1:580731/81‑1 (MQ=255)
cAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACg < 2:580729/81‑1 (MQ=255)
aTCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGa > 2:580773/1‑80 (MQ=255)
aTCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGAc < 1:580732/81‑1 (MQ=255)
gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc > 2:580758/1‑81 (MQ=255)
gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc > 1:580775/1‑81 (MQ=255)
gCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATc > 1:580760/1‑81 (MQ=255)
ccccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATccc < 1:580733/81‑1 (MQ=255)
cccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATcccc < 1:580735/81‑1 (MQ=255)
ccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCg > 1:580777/1‑81 (MQ=255)
ccGTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCg > 2:580780/1‑81 (MQ=255)
gTGAGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGa > 1:580772/1‑81 (MQ=255)
aGCGGCGCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGc < 1:580736/81‑1 (MQ=255)
gcggcgCGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCt < 1:580737/81‑1 (MQ=255)
gcgcGGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTa > 1:580787/1‑81 (MQ=255)
gcgGTGGTCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTAt > 1:580780/1‑80 (MQ=255)
ggtggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCaa > 2:580791/1‑81 (MQ=255)
gtggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAAc < 1:580738/81‑1 (MQ=255)
ggtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACg > 2:580778/1‑80 (MQ=255)
gtCATCAACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGa < 2:580738/81‑1 (MQ=255)
atcaACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAAc < 2:580739/80‑1 (MQ=255)
caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt > 1:580785/1‑81 (MQ=255)
caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt > 2:580770/1‑81 (MQ=255)
caACGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCt > 2:580775/1‑81 (MQ=255)
aaCGAATGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTc < 1:580741/81‑1 (MQ=255)
aaTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGtt > 2:580788/1‑81 (MQ=255)
aTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTc > 1:580766/1‑81 (MQ=255)
aTGGGAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTc < 1:580742/81‑1 (MQ=255)
|
CCGCCAGGAAGAGCATAACAATGATAAAGCCAATCGCCCCCGTGAGCGGCGCGGTGGTCATCAACGAATGGAAATAGTTGCCCATCGGATCCTGATAAACCAGACGCAACGACATCCCCGGATGCTTTATCAACGGAACGCTCAGTTC > NC_000913/3402870‑3403017
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
