Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,446,157 |
G→A |
W682* (TGG→TGA) |
tamB → |
translocation and assembly module subunit TamB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,446,157 | 0 | G | A | 100.0%
| 194.1
/ NA
| 53 | W682* (TGG→TGA) | tamB | translocation and assembly module subunit TamB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/25); total (28/25) |
AGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTAA > NC_000913/4446079‑4446234
|
aGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATc < 1:918990/81‑1 (MQ=255)
ggAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCg > 2:919044/1‑81 (MQ=255)
ttttGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGAc < 2:918991/81‑1 (MQ=255)
tGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCcg > 2:919042/1‑81 (MQ=255)
gATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCcgc > 1:919045/1‑81 (MQ=255)
aTCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCcgc > 2:919053/1‑80 (MQ=255)
tCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGa < 2:918992/81‑1 (MQ=255)
cGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGa > 1:919047/1‑80 (MQ=255)
gCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGata > 2:919035/1‑81 (MQ=255)
gCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGata > 1:919063/1‑81 (MQ=255)
aaaGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCTGGTTGGCCCGTGATCGCTGACCCGCGATAtt > 2:919059/1‑81 (MQ=255)
aaaGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATAtt < 2:918993/81‑1 (MQ=255)
gacgaaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTgcg < 2:918995/78‑1 (MQ=255)
gaagaaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTgcg < 2:918994/81‑1 (MQ=255)
agaaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCt < 1:918996/81‑1 (MQ=255)
gaaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTg > 2:919058/1‑81 (MQ=255)
gaaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTg < 2:918997/81‑1 (MQ=255)
aaCGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTgg < 2:918998/81‑1 (MQ=255)
gAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTAcc < 2:918999/80‑1 (MQ=255)
gAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTAc < 1:919000/79‑1 (MQ=255)
gAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCg > 2:919050/1‑81 (MQ=255)
aaGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCg > 1:919057/1‑80 (MQ=255)
aaGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCg > 2:919049/1‑80 (MQ=255)
gggAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCaa > 2:919037/1‑81 (MQ=255)
gAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATa < 1:919001/81‑1 (MQ=255)
aaCTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATaa < 1:919002/81‑1 (MQ=255)
aGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGc < 2:919003/79‑1 (MQ=255)
aGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCa > 2:919071/1‑80 (MQ=255)
aGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCa > 2:919039/1‑80 (MQ=255)
cAATACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCaaa > 1:919065/1‑80 (MQ=255)
aaTACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCaaaaa < 1:919004/81‑1 (MQ=255)
aaTACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCaaaa > 2:919054/1‑80 (MQ=255)
aTACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCaaaaaa > 2:919077/1‑81 (MQ=255)
tACCCGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAAt < 2:919005/81‑1 (MQ=255)
cccGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCa < 2:919006/81‑1 (MQ=255)
ccGCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAg < 2:919007/81‑1 (MQ=255)
gCTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCa > 2:919062/1‑81 (MQ=255)
cTTCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCAt < 2:919008/81‑1 (MQ=255)
tCCAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCg > 1:919070/1‑81 (MQ=255)
ccAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATc < 1:919009/79‑1 (MQ=255)
ccAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCgg > 2:919068/1‑81 (MQ=255)
cAGACGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCgg < 2:919010/80‑1 (MQ=255)
aCGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAGAATCAGCATCGGGCca > 1:919055/1‑81 (MQ=255)
cGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCcac < 1:919011/81‑1 (MQ=255)
cGCCGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCca > 1:919052/1‑80 (MQ=255)
cGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACAct > 1:919094/1‑81 (MQ=255)
cGGTTGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACAct > 2:919066/1‑81 (MQ=255)
ttGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACActgct > 2:919061/1‑81 (MQ=255)
ttGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACActgct < 1:919012/81‑1 (MQ=255)
tGGCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACActgctg > 1:919064/1‑81 (MQ=255)
ggCCCGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACActgctg < 2:919013/80‑1 (MQ=255)
ccGTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCtt < 2:919014/81‑1 (MQ=255)
gTGATCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTaa > 2:919056/1‑81 (MQ=255)
|
AGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTCGCTGACCCGCGATATTGCGCTGGATTACCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTAA > NC_000913/4446079‑4446234
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
