Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,481,269 |
G→A |
P857S (CCT→TCT) |
valS ← |
valine‑‑tRNA ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,481,269 | 0 | G | A | 100.0%
| 137.4
/ NA
| 38 | P857S (CCT→TCT) | valS | valine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/23); total (15/23) |
GAGGCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCCG > NC_000913/4481192‑4481346
|
gaGGCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGgcagacag > 2:927423/1‑81 (MQ=255)
ggCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGacagca > 1:927419/1‑81 (MQ=255)
gccagccaTCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCAc < 2:927365/81‑1 (MQ=255)
caTCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGa > 1:927418/1‑80 (MQ=255)
aTCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATa > 1:927427/1‑81 (MQ=255)
tCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATAc < 2:927366/81‑1 (MQ=255)
gATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTc < 1:927367/81‑1 (MQ=255)
agcagcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAg < 1:927368/80‑1 (MQ=255)
agcagcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGa > 2:927424/1‑81 (MQ=255)
gcagcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAc < 1:927369/81‑1 (MQ=255)
agcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgc < 1:927370/81‑1 (MQ=255)
agcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgc < 1:927372/81‑1 (MQ=255)
agcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgc < 2:927371/81‑1 (MQ=255)
gcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgcg < 2:927373/81‑1 (MQ=255)
tGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGcc < 2:927374/79‑1 (MQ=255)
tGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGcc < 2:927375/79‑1 (MQ=255)
cACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAggg < 2:927376/81‑1 (MQ=255)
cGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTg > 2:927443/1‑79 (MQ=255)
gatgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAg < 1:927377/81‑1 (MQ=255)
atgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAgg < 2:927378/81‑1 (MQ=255)
tgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGa < 2:927379/81‑1 (MQ=255)
gatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAgg > 2:927433/1‑79 (MQ=255)
atCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGaa < 2:927381/80‑1 (MQ=255)
atCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGaa < 2:927380/80‑1 (MQ=255)
atCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAg > 2:927435/1‑81 (MQ=255)
cGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAg < 1:927382/76‑1 (MQ=255)
gTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACgg > 1:927445/1‑81 (MQ=255)
gTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACgg > 2:927430/1‑81 (MQ=255)
tAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGt < 1:927383/81‑1 (MQ=255)
tAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGt < 1:927384/81‑1 (MQ=255)
aCGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGttt > 2:927436/1‑81 (MQ=255)
aCGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGtt < 2:927385/80‑1 (MQ=255)
cGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGtttt > 2:927449/1‑81 (MQ=255)
gAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCa > 2:927439/1‑81 (MQ=255)
aaaCCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCAt > 2:927453/1‑81 (MQ=255)
aaCCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCAtt < 1:927386/81‑1 (MQ=255)
ggACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTAcg > 1:927437/1‑81 (MQ=255)
ggACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTAcg > 1:927447/1‑81 (MQ=255)
tGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGtt > 1:927440/1‑81 (MQ=255)
tCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCt < 2:927387/81‑1 (MQ=255)
gcaggcagTACGCTGCTATTTTCCAGCCGCGTCAGGCTTTGCCTGAATCCACGCTTTTCATTTCCGCGACGTTCTGCATcc > 2:1051799/1‑81 (MQ=255)
cagacagCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCcg < 2:927388/81‑1 (MQ=255)
|
GAGGCCAGCCATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCCG > NC_000913/4481192‑4481346
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
