Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,481,269 |
G→A |
P857S (CCT→TCT) |
valS ← |
valine‑‑tRNA ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,481,269 | 0 | G | A | 100.0%
| 116.8
/ NA
| 33 | P857S (CCT→TCT) | valS | valine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/17); total (16/17) |
ATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCC > NC_000913/4481202‑4481345
|
aTCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATa > 2:857539/1‑81 (MQ=255)
ggATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACttt < 1:857494/81‑1 (MQ=255)
gATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTc < 1:857495/81‑1 (MQ=255)
aTCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTcc < 2:857495/81‑1 (MQ=255)
cagcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcg < 2:857496/81‑1 (MQ=255)
cagcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcg < 1:857498/81‑1 (MQ=255)
agcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgc > 1:857571/1‑81 (MQ=255)
agcTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGAcgc < 1:857499/81‑1 (MQ=255)
tctGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACgcgc > 1:857539/1‑80 (MQ=255)
ccGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGtt > 1:857561/1‑81 (MQ=255)
cGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGttt < 2:857500/81‑1 (MQ=255)
cGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGttt < 2:857499/81‑1 (MQ=255)
gatgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCa < 2:857501/80‑1 (MQ=255)
gatgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAg > 2:857547/1‑81 (MQ=255)
tgatCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGa < 2:857502/81‑1 (MQ=255)
tCGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCAc > 2:857542/1‑81 (MQ=255)
cGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAGGcc < 2:857504/78‑1 (MQ=255)
cGTAACGGAAACCGGACCTTTGTCATCGGCAGACAGCACGATGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAg < 2:857503/76‑1 (MQ=255)
aaCGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGtt > 1:857545/1‑81 (MQ=255)
aCGGAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGttt < 2:857505/81‑1 (MQ=255)
ggAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGtttt > 1:857547/1‑80 (MQ=255)
ggAAACCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGtttt > 1:857546/1‑80 (MQ=255)
aaaCCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCAt > 2:857506/1‑81 (MQ=255)
aCCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCAttt < 2:857507/81‑1 (MQ=255)
aCCGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCAtt > 2:857557/1‑80 (MQ=255)
cGGACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTAc > 2:857578/1‑81 (MQ=255)
gACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTTATTTAcgc < 1:857509/81‑1 (MQ=255)
gACCTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTAcgc > 1:857560/1‑81 (MQ=255)
cTTTGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGAc < 1:857510/81‑1 (MQ=255)
tGTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGt < 2:857510/80‑1 (MQ=255)
gTCATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTc > 1:857552/1‑81 (MQ=255)
cATCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTg > 2:857566/1‑81 (MQ=255)
aTCGGCAGACAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTg > 1:857563/1‑80 (MQ=255)
ggcagacaGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATc > 1:857565/1‑81 (MQ=255)
ggcagacaGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATc < 2:857511/81‑1 (MQ=255)
gcagacagCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATcc < 1:857513/81‑1 (MQ=255)
|
ATCGGGATCAGCAGCTCTGCACCGTCGATGATCTTCGTAACGGAAACCGGACCTTTGTCATCGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCC > NC_000913/4481202‑4481345
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
