Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
182 |
31.9 |
1789978 |
98.5% |
1763128 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,994,461 |
(T)8→7 |
pseudogene (443/444 nt) |
ygeK ← |
putative DNA‑binding transcriptional regulator YgeK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,994,454 | 0 | T | . | 75.0%
| 16.6
/ 10.8
| 12 | intergenic (+13/+6) | pbl/ygeK | transglycosylase domain‑containing protein Pbl/putative DNA‑binding transcriptional regulator YgeK |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base . (6/3); total (7/5) |
| Fisher's exact test for biased strand distribution p-value = 5.23e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAT > NC_000913/2994380‑2994526
|
aaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCttttttt > 1:505530/1‑81 (MQ=255)
atatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttttttat < 1:505503/81‑3 (MQ=255)
tatataCCTGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCaa < 2:505504/80‑1 (MQ=255)
tatataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCa > 1:505522/1‑80 (MQ=255)
atataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCaaa > 2:505521/1‑80 (MQ=255)
ccAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCa < 2:505505/81‑1 (MQ=255)
ggCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATAc < 1:505506/81‑1 (MQ=255)
aCTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGt > 2:505526/1‑81 (MQ=255)
tCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCa < 1:505507/81‑1 (MQ=255)
aaGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTc > 1:505523/1‑80 (MQ=255)
ttCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATAc > 2:505533/1‑80 (MQ=255)
gAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACAt > 2:505536/1‑81 (MQ=255)
ccTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCt > 1:505534/1‑80 (MQ=255)
aaGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAt < 1:505508/80‑1 (MQ=255)
|
AAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAT > NC_000913/2994380‑2994526
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
