Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,905,487 |
C→T |
intergenic (‑69/‑70) |
queE ← / → yqcG |
putative 7‑carboxy‑7‑deazaguanine synthase QueE/cell envelope stress response protein YqcG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,905,487 | 0 | C | T | 100.0%
| 62.3
/ NA
| 19 | intergenic (‑69/‑70) | queE/yqcG | putative 7‑carboxy‑7‑deazaguanine synthase QueE/cell envelope stress response protein YqcG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/14); total (5/14) |
GTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATT > NC_000913/2905410‑2905556
|
gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATAtt < 1:472525/81‑1 (MQ=255)
gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATAtt < 2:472526/81‑1 (MQ=255)
cATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGtt < 2:472527/80‑1 (MQ=255)
cATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGt < 1:472528/79‑1 (MQ=255)
aTAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTAc < 1:472529/81‑1 (MQ=255)
aGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTAcag > 2:472549/1‑81 (MQ=255)
tgAAGTGGATAATTGTTAATTATTGCAGGTCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGttt < 2:472530/78‑1 (MQ=255)
tgAAGTGGATAATTGTTAATTATTGCAGATCCTGCCAAAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTaa < 2:472531/80‑1 (MQ=255)
aGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGaa < 2:472532/81‑1 (MQ=255)
aGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGaa < 2:472533/81‑1 (MQ=255)
tAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGc > 2:472556/1‑81 (MQ=255)
tAATTGCTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGc > 2:472553/1‑81 (MQ=255)
attGCAGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCg < 2:472534/80‑1 (MQ=255)
aGATCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTtaat > 1:472555/1‑80 (MQ=255)
aGATACTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTtaat > 2:472548/1‑80 (MQ=255)
aTCCTGCCACAACAATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTaataat < 2:472535/81‑1 (MQ=255)
acaacaATCATGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGa < 1:472536/81‑1 (MQ=255)
tGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGtatt < 1:472538/81‑1 (MQ=255)
tGTCTTATTAATATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGtatt < 1:472537/81‑1 (MQ=255)
|
GTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATT > NC_000913/2905410‑2905556
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
