Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 690 31.8 1836723 97.1% 1783458 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,960,513 T→C D639G (GAT→GGT)  recC ← exodeoxyribonuclease V subunit RecC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,960,5130TC100.0% 158.6 / NA 45D639G (GAT→GGT) recCexodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/23);  total (22/23)

ATCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCATCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCATTGT  >  NC_000913/2960435‑2960587
                                                                              |                                                                          
aTCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTtcacca                                                                          <  1:483701/81‑1 (MQ=255)
  cAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACg                                                                        >  2:483768/1‑81 (MQ=255)
   agagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACg                                                                        <  2:483702/80‑1 (MQ=255)
    gagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGc                                                                       >  1:483760/1‑80 (MQ=255)
    gagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCa                                                                      <  1:483703/81‑1 (MQ=255)
      gTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCaa                                                                     >  1:483750/1‑80 (MQ=255)
           aaTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGa                                                               >  1:483744/1‑81 (MQ=255)
            aTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGa                                                               >  1:483764/1‑80 (MQ=255)
            aTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGAc                                                              <  2:483704/81‑1 (MQ=255)
               ttAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGc                                                           <  1:483705/81‑1 (MQ=255)
                tAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCg                                                          >  2:483763/1‑81 (MQ=255)
                     ggTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCAcc                                                     >  2:483766/1‑81 (MQ=255)
                     ggTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCAcc                                                     >  1:483757/1‑81 (MQ=255)
                         cGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGt                                                 >  1:483743/1‑81 (MQ=255)
                          ggCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTc                                                <  1:483706/81‑1 (MQ=255)
                          ggCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTc                                                <  1:483707/81‑1 (MQ=255)
                           gCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCg                                               >  2:483765/1‑81 (MQ=255)
                             gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  2:483709/81‑1 (MQ=255)
                             gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  1:483708/81‑1 (MQ=255)
                             gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGc                                              >  2:483771/1‑80 (MQ=255)
                             gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTGCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  2:483710/81‑1 (MQ=255)
                               gaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCa                                            >  2:483753/1‑80 (MQ=255)
                               gaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCAt                                           <  2:483711/81‑1 (MQ=255)
                                aaaaCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATa                                          <  2:483712/81‑1 (MQ=255)
                                 aaaCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATAc                                         >  1:483756/1‑81 (MQ=255)
                                   aCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTg                                       <  1:483713/81‑1 (MQ=255)
                                   aCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTg                                       <  2:483714/81‑1 (MQ=255)
                                     gctggctgATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTgcg                                     >  1:483740/1‑81 (MQ=255)
                                           tgATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCt                                >  1:483755/1‑80 (MQ=255)
                                            gATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               >  2:483751/1‑80 (MQ=255)
                                            gATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               >  2:483767/1‑80 (MQ=255)
                                             aTACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTaaa                             <  2:483716/81‑1 (MQ=255)
                                             aTACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               <  1:483715/79‑1 (MQ=255)
                                              tACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAAc                            >  1:483775/1‑81 (MQ=255)
                                              tACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAAc                            >  2:483762/1‑81 (MQ=255)
                                                cGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCt                          >  2:483754/1‑81 (MQ=255)
                                                       gATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGc                     <  1:483717/79‑1 (MQ=255)
                                                        aTCCAGACGCTGTGCCAGTTCACCACGCAATGGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGAt                  <  2:483718/81‑1 (MQ=255)
                                                         tCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATa                 <  2:483719/81‑1 (MQ=255)
                                                           cAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAAt               <  1:483720/81‑1 (MQ=255)
                                                            aGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATc              >  2:483761/1‑81 (MQ=255)
                                                                gCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCt          <  2:483722/81‑1 (MQ=255)
                                                                gCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCt          <  2:483721/81‑1 (MQ=255)
                                                                  tgtgCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGc        >  2:483780/1‑81 (MQ=255)
                                                                      ccAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCAtt    <  1:483723/81‑1 (MQ=255)
                                                                        aGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCAttgt  <  1:483724/81‑1 (MQ=255)
                                                                              |                                                                          
ATCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCATCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCATTGT  >  NC_000913/2960435‑2960587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A