Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,028,232 |
C→A |
A371D (GCT→GAT) |
ghxQ → |
guanine/hypoxanthine transporter GhxQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,028,232 | 0 | C | A | 100.0%
| 97.0
/ NA
| 28 | A371D (GCT→GAT) | ghxQ | guanine/hypoxanthine transporter GhxQ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/10); total (18/10) |
TAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATG > NC_000913/3028154‑3028305
|
tAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGatc < 1:496726/81‑1 (MQ=255)
aGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGatct > 2:496762/1‑81 (MQ=255)
tGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGa > 1:496764/1‑81 (MQ=255)
gggggCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGAt > 1:496760/1‑81 (MQ=255)
ggggCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGAt < 1:496727/80‑1 (MQ=255)
gCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTAc < 2:496727/81‑1 (MQ=255)
aTTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAgg > 2:496765/1‑81 (MQ=255)
ttCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGt > 1:496759/1‑81 (MQ=255)
ttCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGt > 1:496756/1‑81 (MQ=255)
cTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGttt < 1:496729/80‑1 (MQ=255)
cTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGttt < 2:496729/80‑1 (MQ=255)
aaTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGAt > 2:496756/1‑81 (MQ=255)
aTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATg > 1:496761/1‑81 (MQ=255)
aTTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATg < 2:496730/81‑1 (MQ=255)
ttCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGt < 1:496732/81‑1 (MQ=255)
cTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGtt > 2:496753/1‑80 (MQ=255)
tATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACg > 1:496782/1‑81 (MQ=255)
tATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACg > 1:496767/1‑81 (MQ=255)
cTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAg < 2:496732/81‑1 (MQ=255)
tCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCt < 2:496733/80‑1 (MQ=255)
aTTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGa > 2:496768/1‑81 (MQ=255)
cTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGAtt > 1:496779/1‑81 (MQ=255)
gTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGAtttt < 2:496734/80‑1 (MQ=255)
gTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTa > 1:496768/1‑81 (MQ=255)
tACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt > 1:496770/1‑81 (MQ=255)
tACGCCACTGCACCCGATCTGATGTACGCAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt > 2:496770/1‑81 (MQ=255)
aCGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt > 1:496765/1‑80 (MQ=255)
cACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGc > 1:496773/1‑81 (MQ=255)
cACTGCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGc < 2:496735/81‑1 (MQ=255)
gCACCCGATCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTAtg > 2:496789/1‑81 (MQ=255)
|
TAGTGGGGGCGTTATTCCTGTTAATTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATG > NC_000913/3028154‑3028305
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
