Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,077,875 |
C→T |
A329T (GCT→ACT) |
cmtA ← |
mannitol‑specific PTS enzyme IICB component CmtA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,077,875 | 0 | C | T | 100.0%
| 140.6
/ NA
| 39 | A329T (GCT→ACT) | cmtA | mannitol‑specific PTS enzyme IICB component CmtA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/18); total (21/18) |
CATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGT > NC_000913/3077797‑3077949
|
cATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTaa < 1:507485/81‑1 (MQ=255)
tGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAg > 1:507530/1‑80 (MQ=255)
ggCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAgg < 2:507485/80‑1 (MQ=255)
actgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGacac > 1:507531/1‑79 (MQ=255)
ctgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAg < 1:507487/81‑1 (MQ=255)
tgaGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAgg > 1:507551/1‑81 (MQ=255)
aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc > 2:507534/1‑81 (MQ=255)
aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc < 1:507488/81‑1 (MQ=255)
aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc > 2:507542/1‑81 (MQ=255)
aaaCTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGtacc > 1:507553/1‑81 (MQ=255)
ttCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTaa > 1:507540/1‑80 (MQ=255)
cGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAaca < 1:507489/80‑1 (MQ=255)
ctcCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCgg > 2:507536/1‑80 (MQ=255)
ccGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCaa > 2:507557/1‑81 (MQ=255)
gTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAAtt > 1:507545/1‑81 (MQ=255)
ttCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGt > 1:507554/1‑81 (MQ=255)
cACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGc < 2:507489/81‑1 (MQ=255)
aCCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGcc < 2:507490/81‑1 (MQ=255)
cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg < 1:507494/81‑1 (MQ=255)
cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg < 1:507492/81‑1 (MQ=255)
cGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAg < 1:507493/81‑1 (MQ=255)
gTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAgg < 2:507494/81‑1 (MQ=255)
tttttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGaa > 1:507548/1‑81 (MQ=255)
tttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAAc > 1:507539/1‑80 (MQ=255)
ttCCATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACg < 2:507495/80‑1 (MQ=255)
ccATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAg < 2:507497/80‑1 (MQ=255)
ccATCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAg < 1:507497/80‑1 (MQ=255)
aTCTTCAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCt < 1:507499/81‑1 (MQ=255)
cAGTATCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCgg > 2:507540/1‑81 (MQ=255)
taacAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt < 2:507499/78‑1 (MQ=255)
aTCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt > 1:507561/1‑80 (MQ=255)
tCAGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGt > 1:507574/1‑79 (MQ=255)
aGCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGt < 2:507500/81‑1 (MQ=255)
gCGAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTg > 2:507564/1‑81 (MQ=255)
gAAGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGc > 1:507552/1‑80 (MQ=255)
aaGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCa < 2:507501/81‑1 (MQ=255)
aGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAg > 2:507537/1‑81 (MQ=255)
aGTAATAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAg > 2:507566/1‑81 (MQ=255)
aaTAGTAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAgat > 2:507543/1‑78 (MQ=255)
|
CATTGGCTGACTGAGCAAACTCATCTTCGCTCTCCGTTTCCACCGTTTTTTCCATCTTCAGTATCAGCGAAGTAATAGCAAAGGACACCAGGGTACCTACCGTAACACCGGCAATTGTCGCCAGGAACGAGCCTTTCGGCGTCAGTGCCAGGT > NC_000913/3077797‑3077949
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
