Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,124,738 |
G→A |
A241V (GCC→GTC) |
glcF ← |
glycolate dehydrogenase, putative iron‑sulfur subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,124,738 | 0 | G | A | 100.0%
| 106.9
/ NA
| 30 | A241V (GCC→GTC) | glcF | glycolate dehydrogenase, putative iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/19); total (11/19) |
CATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATT > NC_000913/3124664‑3124811
|
cATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTg < 1:517542/81‑1 (MQ=255)
tttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAAt > 1:517578/1‑81 (MQ=255)
ttCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAAt < 1:517543/80‑1 (MQ=255)
gCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCgg < 1:517544/81‑1 (MQ=255)
cATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCggg > 1:517571/1‑81 (MQ=255)
aTCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGc > 1:517582/1‑81 (MQ=255)
tCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGc > 1:517586/1‑80 (MQ=255)
cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc < 1:517548/80‑1 (MQ=255)
cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc < 1:517545/80‑1 (MQ=255)
cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGccacc < 2:517545/80‑1 (MQ=255)
cccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGcc < 2:517546/77‑1 (MQ=255)
ccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAg < 1:517549/81‑1 (MQ=255)
ccATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAg < 2:517549/81‑1 (MQ=255)
aCTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCAt > 2:517587/1‑81 (MQ=255)
ctTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCaa > 1:517577/1‑81 (MQ=255)
ttGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAata > 1:517585/1‑81 (MQ=255)
cAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAAtattat < 2:517550/81‑1 (MQ=255)
aaaCGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATattatt < 2:517551/81‑1 (MQ=255)
aaCGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTg > 2:517586/1‑81 (MQ=255)
ccgcAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGcc < 1:517553/81‑1 (MQ=255)
cAGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGt < 2:517553/81‑1 (MQ=255)
aGCCGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTg < 2:517554/81‑1 (MQ=255)
cGCTGGCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGc > 2:517589/1‑79 (MQ=255)
gCGGTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCt > 2:517588/1‑81 (MQ=255)
ggTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCttt < 1:517556/81‑1 (MQ=255)
gTTTGCAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTtc < 1:517557/81‑1 (MQ=255)
cAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCt < 2:517558/80‑1 (MQ=255)
cAAAATTGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCt < 1:517558/80‑1 (MQ=255)
tGCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCAt > 2:517590/1‑80 (MQ=255)
gCCTCGACACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCAtt < 2:517559/80‑1 (MQ=255)
|
CATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATTATTGCGCGCCCGTGCCAGCCCTTTCTCCTGCGCATT > NC_000913/3124664‑3124811
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
