Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,193,105 |
G→A |
R81H (CGC→CAC) |
yqiK → |
protein YqiK |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,193,105 | 0 | G | A | 100.0%
| 106.2
/ NA
| 30 | R81H (CGC→CAC) | yqiK | protein YqiK |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/11); total (19/11) |
GCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCGCTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAA > NC_000913/3193033‑3193181
|
gcgcAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCa > 1:532309/1‑81 (MQ=255)
gcAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCAtt > 2:532315/1‑81 (MQ=255)
ttCATGAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGa > 1:532318/1‑78 (MQ=255)
gAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCg > 2:532310/1‑80 (MQ=255)
gAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCg > 1:532315/1‑80 (MQ=255)
gAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCg > 1:532320/1‑80 (MQ=255)
tCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGcgcg < 1:532276/81‑1 (MQ=255)
tCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGcgcg < 2:532274/81‑1 (MQ=255)
ccccATCAATATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGt > 2:532309/1‑81 (MQ=255)
aaTATGAATACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATg > 2:532331/1‑79 (MQ=255)
aaTACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTagtagt < 1:532279/80‑1 (MQ=255)
aaTACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTc < 1:532277/81‑1 (MQ=255)
aaTACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATCTAGTAGTc < 1:532278/81‑1 (MQ=255)
aTACTCTGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCg < 1:532280/81‑1 (MQ=255)
tGAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGctttct > 1:532330/1‑81 (MQ=255)
gAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGctttctt > 1:532336/1‑81 (MQ=255)
gAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGctttctt > 2:532333/1‑81 (MQ=255)
gAAGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGctttctt > 1:532322/1‑81 (MQ=255)
aaGCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGctttctt < 1:532281/80‑1 (MQ=255)
gCTGGAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTtgt < 2:532281/81‑1 (MQ=255)
ggAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCg > 1:532327/1‑81 (MQ=255)
ggAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCg > 2:532322/1‑81 (MQ=255)
gAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGc < 2:532282/79‑1 (MQ=255)
gAAGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCgg > 2:532327/1‑81 (MQ=255)
aaGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCggg > 2:532332/1‑81 (MQ=255)
aGTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGt < 2:532283/81‑1 (MQ=255)
gTCAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTa < 2:532284/81‑1 (MQ=255)
cAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTaa > 2:532328/1‑80 (MQ=255)
cAGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTaa > 2:532334/1‑80 (MQ=255)
aGCCACTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTaaaa > 1:532326/1‑81 (MQ=255)
|
GCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAGCTGGAAGTCAGCCGCTCAACCATTGATAGCCTGATTACGAAAGATCGTATGCGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAA > NC_000913/3193033‑3193181
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
