Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,385,853 |
(T)8→9 |
intergenic (+52/+4) |
yhcN → / ← yhcO |
DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,385,845 | 1 | . | T | 100.0%
| 80.4
/ NA
| 23 | intergenic (+44/+12) | yhcN/yhcO | DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (11/12); total (11/12) |
GACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAA > NC_000913/3385766‑3385919
|
gACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑t > 1:576636/1‑81 (MQ=255)
gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑ttttttttt > 1:576643/1‑80 (MQ=255)
gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑ttttttt < 2:576595/79‑1 (MQ=255)
aCGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑tttttttttgc > 1:576631/1‑78 (MQ=255)
aCGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑tttttttttgc < 2:576596/81‑4 (MQ=255)
cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑tttttttttgcg > 2:576633/1‑77 (MQ=255)
ggCTGAACTGTACAATTAAACCCTCACCGTCTTTTCCGACGATATTGCCTCCGGTTCGGGGGCTTTTTTTTTGCGCTAATg > 2:576643/1‑81 (MQ=255)
tGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATTAc > 2:576634/1‑80 (MQ=255)
agcTGTACAAGTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCAGTTCGGGGGCTTTTTTTTTGCGCTGATGACGaa < 2:576597/79‑1 (MQ=255)
tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTa < 1:576599/81‑1 (MQ=255)
cAAATAAACCCTCATCGTCTTGTACGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACGTTaa < 2:576599/80‑1 (MQ=255)
aaaTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCTCTAATGACGAACATTaaaa > 2:576631/1‑81 (MQ=255)
aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGc > 2:576639/1‑80 (MQ=255)
aaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCaa < 1:576601/81‑1 (MQ=255)
aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCt > 1:576627/1‑81 (MQ=255)
cGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc > 1:576633/1‑81 (MQ=255)
gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc < 2:576602/81‑1 (MQ=255)
gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc < 1:576602/81‑1 (MQ=255)
tCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa < 2:576603/81‑1 (MQ=255)
ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc > 1:576629/1‑81 (MQ=255)
gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc > 1:576646/1‑81 (MQ=255)
cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCctc < 2:576604/81‑1 (MQ=255)
gaTATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCctct < 1:576606/81‑1 (MQ=255)
ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGcc < 1:576608/80‑1 (MQ=255)
ttGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt < 2:576606/81‑1 (MQ=255)
gTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa > 2:576644/1‑79 (MQ=255)
tCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg > 2:576638/1‑80 (MQ=255)
gggggCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCaa > 2:576656/1‑81 (MQ=255)
gggggCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa < 2:576608/80‑1 (MQ=255)
|
GACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAA > NC_000913/3385766‑3385919
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
