Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,469,803 |
A→G |
C17C (TGT→TGC) |
chiA ← |
endochitinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,469,803 | 0 | A | G | 100.0%
| 55.5
/ NA
| 17 | C17C (TGT→TGC) | chiA | endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (5/12); total (5/12) |
CATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACG > NC_000913/3469727‑3469870
|
cATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAgcaca < 2:596401/81‑1 (MQ=255)
tCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACAcc < 2:596402/81‑1 (MQ=255)
aaTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGccc > 2:596430/1‑81 (MQ=255)
aTAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGcccc < 2:596403/81‑1 (MQ=255)
aCCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCAt < 2:596404/80‑1 (MQ=255)
cTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATAc > 1:596425/1‑80 (MQ=255)
tttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCAt < 2:596405/81‑1 (MQ=255)
tttattACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCAt < 2:596406/81‑1 (MQ=255)
cATGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTc < 1:596408/81‑1 (MQ=255)
aTGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCa > 1:596430/1‑81 (MQ=255)
aTGCTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCa < 2:596408/81‑1 (MQ=255)
cTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCAtt < 1:596411/80‑1 (MQ=255)
cTTCCATTGCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCAtt < 2:596409/80‑1 (MQ=255)
gCCAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCt > 2:596428/1‑81 (MQ=255)
ccAATGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCtt < 1:596412/81‑1 (MQ=255)
aaTGCTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTtgt < 1:596413/81‑1 (MQ=255)
cTGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACg < 2:596413/81‑1 (MQ=255)
tGGCAGAGCGGAGCACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACg > 2:596433/1‑80 (MQ=255)
|
CATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACG > NC_000913/3469727‑3469870
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
