Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,497,365 |
G→A |
M121I (ATG→ATA) |
nirC → |
nitrite transporter NirC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,497,365 | 0 | G | A | 100.0%
| 125.5
/ NA
| 35 | M121I (ATG→ATA) | nirC | nitrite transporter NirC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/17); total (18/17) |
ATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTAA > NC_000913/3497288‑3497436
|
aTCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATActc > 2:603189/1‑81 (MQ=255)
tCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATActct < 2:603147/81‑1 (MQ=255)
cAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCta > 2:603203/1‑81 (MQ=255)
aGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCta < 2:603148/80‑1 (MQ=255)
cGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTg > 1:603207/1‑81 (MQ=255)
gggCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTgg > 1:603212/1‑81 (MQ=255)
ggCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTggg < 1:603150/81‑1 (MQ=255)
aaaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcg > 1:603206/1‑81 (MQ=255)
aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg > 1:603209/1‑81 (MQ=255)
aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg > 1:603221/1‑81 (MQ=255)
aaTGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGggcgg > 2:603216/1‑81 (MQ=255)
gtgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGcggcgg > 1:603223/1‑81 (MQ=255)
tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGcggcgg < 2:603152/80‑1 (MQ=255)
tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGt < 2:603151/81‑1 (MQ=255)
tgGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGt < 2:603150/81‑1 (MQ=255)
aaTCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCct > 2:603213/1‑81 (MQ=255)
ccGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCg < 1:603154/81‑1 (MQ=255)
gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCgg > 2:603219/1‑80 (MQ=255)
gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGt < 2:603154/81‑1 (MQ=255)
gCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGt < 1:603156/81‑1 (MQ=255)
cAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTa < 2:603156/81‑1 (MQ=255)
ccTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATa < 2:603157/81‑1 (MQ=255)
gggTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCAt > 1:603208/1‑81 (MQ=255)
ggTAACCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATc < 1:603159/81‑1 (MQ=255)
aaCCTGGTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGtt > 1:603218/1‑81 (MQ=255)
ggTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTc > 2:603210/1‑81 (MQ=255)
ggTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTc > 1:603222/1‑81 (MQ=255)
gTCGGTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCGCTcc < 2:603159/81‑1 (MQ=255)
ggTTCCGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTc < 2:603160/81‑1 (MQ=255)
cGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCTTCGCGCt < 2:603161/81‑1 (MQ=255)
cGTCTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCgcgc < 1:603163/80‑1 (MQ=255)
cTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc > 2:603214/1‑81 (MQ=255)
cTTCGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc > 2:603266/1‑81 (MQ=255)
cGTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGc < 2:603163/78‑1 (MQ=255)
gTTGCCATACTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTaa > 2:603212/1‑80 (MQ=255)
|
ATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTTCACTCCGTCGCGCTGGCTAA > NC_000913/3497288‑3497436
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
