Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,565,112 |
G→A |
G490G (GGC→GGT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,565,112 | 0 | G | A | 100.0%
| 143.5
/ NA
| 40 | G490G (GGC→GGT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/20); total (20/20) |
GCGTGATTAACCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGT > NC_000913/3565038‑3565189
|
gCGTGATTAACCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAgg > 2:620536/1‑81 (MQ=255)
gCGTGATTAACCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAgg > 1:620536/1‑81 (MQ=255)
aCCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTcc < 2:620483/81‑1 (MQ=255)
aCCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTc > 2:620558/1‑80 (MQ=255)
cATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAg > 1:620549/1‑81 (MQ=255)
gggAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCAc < 1:620485/80‑1 (MQ=255)
aaaTCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCt > 1:620550/1‑81 (MQ=255)
aaaTCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCt > 1:620546/1‑81 (MQ=255)
ttgttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCTGGTGTTCGTCCAGCACGGCTGAAAGCGATgg > 2:620542/1‑81 (MQ=255)
ttgttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATgg > 2:620539/1‑81 (MQ=255)
ttgCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGtt < 1:620486/81‑1 (MQ=255)
gCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTc < 2:620486/80‑1 (MQ=255)
cTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCTATGGGTTCGCTAc < 1:620489/81‑1 (MQ=255)
cTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTAc < 2:620487/81‑1 (MQ=255)
aTTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgc > 1:620570/1‑81 (MQ=255)
aaGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgcca < 2:620489/80‑1 (MQ=255)
aGCAGGCTAAGTTCGGTGCGCCAGTTACTACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgccag < 1:620491/80‑1 (MQ=255)
aGCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgccagc > 1:620571/1‑81 (MQ=255)
gCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCATCACGGCTGAAAGCGATGGGTTCGCTACCgccagc > 1:620562/1‑80 (MQ=255)
gCAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgccagcc < 2:620491/81‑1 (MQ=255)
cAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgccagcca < 1:620494/81‑1 (MQ=255)
cAGGCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCgccagcca < 2:620492/81‑1 (MQ=255)
ggCTAAGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCcagccagc > 2:620556/1‑81 (MQ=255)
aGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAcgc > 2:620575/1‑81 (MQ=255)
aGGTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGAc < 1:620495/79‑1 (MQ=255)
gTCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACgcgg < 1:620496/81‑1 (MQ=255)
tCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACgcggc < 2:620497/81‑1 (MQ=255)
tCGGTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACgcggc < 1:620497/81‑1 (MQ=255)
ggTGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGt > 1:620553/1‑81 (MQ=255)
tGCGCCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTca > 2:620554/1‑81 (MQ=255)
cgcCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTcac < 2:620499/80‑1 (MQ=255)
cgcCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTcac < 1:620499/80‑1 (MQ=255)
gcCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCacac > 2:620553/1‑81 (MQ=255)
gcCAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCacac > 2:620571/1‑81 (MQ=255)
ccAGTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACAcc < 2:620500/81‑1 (MQ=255)
gTTACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACcgtt < 1:620502/81‑1 (MQ=255)
ttACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACcgttg > 1:620566/1‑81 (MQ=255)
ttACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACcgttg < 1:620503/81‑1 (MQ=255)
ttACGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACcgttg > 2:620580/1‑81 (MQ=255)
aCGACCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACcgttggt > 2:620560/1‑81 (MQ=255)
|
GCGTGATTAACCATTGGGAAATCACAGTGTTGTTGCAGCTCATTAAGCAGGCTAAGGTCGGTGCGCCAGTTACGGCCCAGGTGTTCGTCCAGCACGGCTGAAAGCGATGGGTTCGCTACCGCCAGCCAGCGACGCGGCGTCACACCGTTGGT > NC_000913/3565038‑3565189
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
