Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,571,289 |
Δ1 bp |
coding (31/1974 nt) |
glgX ← |
limit dextrin alpha‑1,6‑glucohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,571,286 | 0 | G | . | 100.0%
| 165.0
/ NA
| 39 | coding (34/1974 nt) | glgX | limit dextrin alpha‑1,6‑glucohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (18/21); total (18/21) |
GTTCTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGAGGGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAGCGGTGGTAGCG > NC_000913/3571217‑3571362
|
gTTCTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGttt < 1:622143/81‑1 (MQ=255)
gTTCTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGttt < 2:622143/81‑1 (MQ=255)
ttCTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTg > 2:622214/1‑81 (MQ=255)
cTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGc < 1:622145/80‑1 (MQ=255)
cTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGc < 2:622145/80‑1 (MQ=255)
aCCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCaa > 1:622205/1‑81 (MQ=255)
cccGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGcc < 2:622146/78‑1 (MQ=255)
cccGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAAt < 2:622147/81‑1 (MQ=255)
gCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGc < 1:622149/81‑1 (MQ=255)
cGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAg > 2:622222/1‑81 (MQ=255)
cGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAg > 1:622213/1‑81 (MQ=255)
gCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGtt > 1:622204/1‑81 (MQ=255)
gCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGtt < 2:622149/81‑1 (MQ=255)
cATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTtg < 1:622151/81‑1 (MQ=255)
aGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTgtgt > 1:622208/1‑80 (MQ=255)
aGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTc < 1:622152/81‑1 (MQ=255)
cGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCAt < 2:622152/81‑1 (MQ=255)
agaAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCt > 2:622202/1‑81 (MQ=255)
agaAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCt < 2:622153/81‑1 (MQ=255)
aaGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCt > 2:622223/1‑81 (MQ=255)
gtgtGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTcc < 1:622155/81‑1 (MQ=255)
tgtgAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTccc < 2:622155/81‑1 (MQ=255)
tgAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCg < 2:622156/80‑1 (MQ=255)
gAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGaa > 2:622208/1‑81 (MQ=255)
gAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGaa > 2:622216/1‑81 (MQ=255)
gACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAAccagc > 2:622210/1‑80 (MQ=255)
gACGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAAccagc < 2:622157/80‑1 (MQ=255)
aCGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAAccagcc < 2:622158/80‑1 (MQ=255)
aCGCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAAccagc > 2:622213/1‑79 (MQ=255)
gCCCTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTCGCCAATGGCGAGTTGTGTCATTCTGCCTCCTGAAccagc > 2:622219/1‑77 (MQ=255)
ccTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGa > 2:622235/1‑79 (MQ=255)
ccTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATa < 2:622159/81‑1 (MQ=255)
cTGACCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTATGCCCCCCGAACCAGCCAGATAg > 2:622228/1‑81 (MQ=255)
aCCGTCGTAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGt < 1:622161/79‑1 (MQ=255)
gtcgtAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGc > 2:622215/1‑79 (MQ=255)
tcgtAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAg > 2:622236/1‑81 (MQ=255)
gtAATGCGCGCCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAGc < 2:622161/80‑1 (MQ=255)
cgcCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAGCGGTGGTAGc < 2:622162/81‑1 (MQ=255)
gcCGA‑GGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAGCGGTGGTAGCg > 2:622226/1‑81 (MQ=255)
|
GTTCTACCCGCTCGGCATGAGCGGAGAAAAGTGTGAAGTTGACGCCCTGACCGTCGTAATGCGCGCCGAGGGGAGCGGGTTTGCCAATGGCGAGTTGTGTCATTCTGCCTCCCGAACCAGCCAGATAGTGGCCAGCGGTGGTAGCG > NC_000913/3571217‑3571362
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
