Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,425,249 |
C→T |
R44R (CGC→CGT) |
rpsF → |
30S ribosomal subunit protein S6 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,425,249 | 0 | C | T | 100.0%
| 134.5
/ NA
| 37 | R44R (CGC→CGT) | rpsF | 30S ribosomal subunit protein S6 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/14); total (23/14) |
GGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGT > NC_000913/4425175‑4425326
|
ggCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAg > 2:844500/1‑81 (MQ=255)
ggCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAg > 1:844529/1‑81 (MQ=255)
gCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGc > 2:844503/1‑81 (MQ=255)
aTCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCt > 1:844519/1‑81 (MQ=255)
aTCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCt < 2:844468/81‑1 (MQ=255)
gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc > 2:844511/1‑81 (MQ=255)
gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc < 2:844470/81‑1 (MQ=255)
gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc < 2:844469/81‑1 (MQ=255)
acacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc > 2:844534/1‑80 (MQ=255)
acacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc > 1:844511/1‑80 (MQ=255)
cacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaa > 1:844534/1‑81 (MQ=255)
cacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc < 1:844472/79‑1 (MQ=255)
ctgctgCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaac > 1:844510/1‑80 (MQ=255)
gctgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACaaa > 1:844525/1‑81 (MQ=255)
ctgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaacaa < 1:844473/79‑1 (MQ=255)
tgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACt > 1:844523/1‑81 (MQ=255)
aCTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGcaca > 1:844518/1‑79 (MQ=255)
aCTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGcaca > 2:844512/1‑79 (MQ=255)
aGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTa < 1:844474/81‑1 (MQ=255)
gAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTAc > 2:844516/1‑81 (MQ=255)
gAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTAc < 2:844474/81‑1 (MQ=255)
gCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTc > 1:844533/1‑81 (MQ=255)
gCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTc < 1:844476/81‑1 (MQ=255)
aaGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCt > 1:844527/1‑80 (MQ=255)
aaGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCt > 1:844526/1‑80 (MQ=255)
aTCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCtgat > 1:844521/1‑80 (MQ=255)
tCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCtgatga < 2:844476/81‑1 (MQ=255)
cACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAAt > 1:844537/1‑81 (MQ=255)
tCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTg > 2:844537/1‑80 (MQ=255)
ggAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGc > 2:844535/1‑81 (MQ=255)
gAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGaa < 2:844477/78‑1 (MQ=255)
aaGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTc < 2:844478/81‑1 (MQ=255)
aCTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCg > 1:844540/1‑80 (MQ=255)
aCTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCg > 2:844531/1‑80 (MQ=255)
gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAgg < 2:844480/80‑1 (MQ=255)
gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAgg < 1:844482/80‑1 (MQ=255)
gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAg < 2:844479/79‑1 (MQ=255)
ccgtcgTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGt < 2:844483/81‑1 (MQ=255)
ccgtcgTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGt < 1:844483/81‑1 (MQ=255)
|
GGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGT > NC_000913/4425175‑4425326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
