Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,321,337 |
G→A |
N16N (AAC→AAT) |
trpD ← |
anthranilate synthase subunit TrpD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,321,337 | 0 | G | A | 100.0%
| 121.7
/ NA
| 35 | N16N (AAC→AAT) | trpD | anthranilate synthase subunit TrpD |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/15); total (20/15) |
TCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT > NC_000913/1321260‑1321413
|
tCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTg > 2:235716/1‑81 (MQ=255)
tGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAagag > 1:235705/1‑81 (MQ=255)
gcCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTc > 2:235703/1‑80 (MQ=255)
atatGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGAtatta > 1:235712/1‑81 (MQ=255)
tGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATc < 2:235671/80‑1 (MQ=255)
gCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGagcag > 1:235719/1‑81 (MQ=255)
cGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGagcagc < 1:235672/81‑1 (MQ=255)
ggTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAgcagca < 1:235673/81‑1 (MQ=255)
aTCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATg < 2:235674/81‑1 (MQ=255)
tCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGGGCAGCAGAATGt < 2:235675/81‑1 (MQ=255)
accacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGt > 1:235709/1‑79 (MQ=255)
accacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTc < 2:235676/80‑1 (MQ=255)
cGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCa > 1:235706/1‑81 (MQ=255)
gTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt < 2:235677/81‑1 (MQ=255)
gTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt > 1:235722/1‑81 (MQ=255)
ttATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt > 2:235721/1‑80 (MQ=255)
tATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt > 1:235708/1‑79 (MQ=255)
gCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGa > 2:235713/1‑80 (MQ=255)
cccATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGaaa < 1:235678/81‑1 (MQ=255)
cccATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGa > 2:235717/1‑79 (MQ=255)
cATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGt > 1:235729/1‑81 (MQ=255)
aTTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtc > 2:235718/1‑81 (MQ=255)
aTTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtc < 1:235679/81‑1 (MQ=255)
gctgcGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtctc < 2:235680/80‑1 (MQ=255)
gctgcGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTcc > 1:235714/1‑81 (MQ=255)
cgcAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCtgtg < 1:235681/81‑1 (MQ=255)
cgcAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCtgtg > 2:235715/1‑81 (MQ=255)
cAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCa < 2:235682/81‑1 (MQ=255)
cTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga > 1:235733/1‑81 (MQ=255)
gATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg > 2:235720/1‑81 (MQ=255)
gATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg > 1:235699/1‑81 (MQ=255)
aTCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg < 2:235683/80‑1 (MQ=255)
ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgcgc < 2:235685/78‑1 (MQ=255)
ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGt < 1:235686/81‑1 (MQ=255)
ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGt > 2:235736/1‑81 (MQ=255)
|
TCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT > NC_000913/1321260‑1321413
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
