Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,353,437 |
A→T |
T64T (ACT→ACA) |
sapD ← |
putrescine ABC exporter ATP binding protein SapD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,353,437 | 0 | A | T | 100.0%
| 97.7
/ NA
| 28 | T64T (ACT→ACA) | sapD | putrescine ABC exporter ATP binding protein SapD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/17); total (11/17) |
TTATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACCAA > NC_000913/1353360‑1353513
|
ttATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTa < 2:240737/81‑1 (MQ=255)
aTGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTaa > 2:240769/1‑80 (MQ=255)
gCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACg > 2:240772/1‑81 (MQ=255)
cAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGcc < 2:240738/81‑1 (MQ=255)
aaCCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCa < 2:240739/81‑1 (MQ=255)
ttGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCt > 2:240785/1‑81 (MQ=255)
cgcCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCttt < 1:240740/80‑1 (MQ=255)
ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc > 2:240775/1‑81 (MQ=255)
ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc < 2:240741/81‑1 (MQ=255)
cgcgTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAcc < 2:240742/80‑1 (MQ=255)
cgTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCca > 2:240787/1‑81 (MQ=255)
cGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACaa < 1:240743/80‑1 (MQ=255)
ggAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAAt < 1:240744/81‑1 (MQ=255)
ggAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAAt < 1:240745/81‑1 (MQ=255)
gcagcaAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCg < 1:240746/81‑1 (MQ=255)
aaTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATc > 2:240781/1‑80 (MQ=255)
aaTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCa < 2:240747/81‑1 (MQ=255)
tatCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAActct < 1:240748/81‑1 (MQ=255)
atcatcAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCtt > 1:240793/1‑81 (MQ=255)
tcatcaAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCtt < 2:240749/80‑1 (MQ=255)
aaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGaa < 1:240750/81‑1 (MQ=255)
aaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGaa > 1:240779/1‑81 (MQ=255)
aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc < 2:240752/81‑1 (MQ=255)
aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc < 2:240753/81‑1 (MQ=255)
aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc < 2:240751/81‑1 (MQ=255)
gCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCg > 2:240782/1‑80 (MQ=255)
cATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGa > 2:240783/1‑81 (MQ=255)
ggTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc > 1:240794/1‑81 (MQ=255)
cagctgTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACcaa > 1:240786/1‑81 (MQ=255)
|
TTATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACCAA > NC_000913/1353360‑1353513
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
