Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,353,437 |
A→T |
T64T (ACT→ACA) |
sapD ← |
putrescine ABC exporter ATP binding protein SapD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,353,437 | 0 | A | T | 100.0%
| 70.8
/ NA
| 20 | T64T (ACT→ACA) | sapD | putrescine ABC exporter ATP binding protein SapD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (6/14); total (6/14) |
ATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAC > NC_000913/1353362‑1353510
|
aTGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTaa < 2:260789/80‑1 (MQ=255)
tGGCCAACCAGTTTGCGCCGTTCGCGTGCGGGGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAaca < 2:260790/81‑1 (MQ=255)
gCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACg < 1:260791/81‑1 (MQ=255)
ccAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGc < 1:260792/81‑1 (MQ=255)
aaCCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCa < 1:260793/81‑1 (MQ=255)
tttGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATc < 1:260794/81‑1 (MQ=255)
tGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCt < 2:260795/80‑1 (MQ=255)
ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc < 1:260796/81‑1 (MQ=255)
agaCGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCt > 2:260827/1‑81 (MQ=255)
aTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCaa < 1:260797/81‑1 (MQ=255)
atatCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAActc < 1:260798/81‑1 (MQ=255)
aaaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCgg < 2:260799/80‑1 (MQ=255)
aCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAcc > 1:260825/1‑81 (MQ=255)
aCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAcc < 2:260800/81‑1 (MQ=255)
cATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGa > 1:260829/1‑81 (MQ=255)
aTACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAt > 2:260824/1‑81 (MQ=255)
tACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAtt < 1:260801/81‑1 (MQ=255)
aCGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGAtt > 2:260822/1‑80 (MQ=255)
ggTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc < 1:260802/81‑1 (MQ=255)
gTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc > 2:260826/1‑80 (MQ=255)
|
ATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAC > NC_000913/1353362‑1353510
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
