Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,411,109 |
A→T |
D280E (GAT→GAA) |
ttcA ← |
tRNA cytosine(32) 2‑sulfurtransferase TtcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,411,109 | 0 | A | T | 100.0%
| 110.6
/ NA
| 33 | D280E (GAT→GAA) | ttcA | tRNA cytosine(32) 2‑sulfurtransferase TtcA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (21/12); total (21/12) |
GCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAT > NC_000913/1411036‑1411182
|
gCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACg > 1:250527/1‑81 (MQ=255)
tAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc > 2:250510/1‑78 (MQ=255)
tAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAg < 1:250470/81‑1 (MQ=255)
aCTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc > 2:250535/1‑76 (MQ=255)
cTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGAt > 1:250503/1‑80 (MQ=255)
cATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc > 2:250531/1‑81 (MQ=255)
ccAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcccc > 2:250505/1‑81 (MQ=255)
cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAccccc < 1:250471/81‑1 (MQ=255)
cTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGtt < 1:250472/81‑1 (MQ=255)
aTTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAAc < 2:250473/81‑1 (MQ=255)
cATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACt < 1:250474/80‑1 (MQ=255)
cttcttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACtt > 2:250508/1‑78 (MQ=255)
cttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGa < 1:250475/79‑1 (MQ=255)
tctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCg > 1:250513/1‑81 (MQ=255)
ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAc < 1:250476/78‑1 (MQ=255)
ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt > 2:250507/1‑81 (MQ=255)
tGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTg > 1:250532/1‑81 (MQ=255)
ggCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg > 2:250518/1‑81 (MQ=255)
gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTggg > 2:250515/1‑81 (MQ=255)
gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg < 1:250477/80‑1 (MQ=255)
aGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGc > 1:452271/1‑81 (MQ=255)
cACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCt > 1:250529/1‑81 (MQ=255)
cGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCttt < 1:250478/81‑1 (MQ=255)
ggTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGaaa > 1:250525/1‑81 (MQ=255)
gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg > 2:250516/1‑80 (MQ=255)
tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg < 1:250479/81‑1 (MQ=255)
ggATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt < 1:250480/81‑1 (MQ=255)
ggATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt > 2:250538/1‑81 (MQ=255)
gATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGtt < 2:250481/81‑1 (MQ=255)
gATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGtt > 1:250517/1‑81 (MQ=255)
ctctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTgg > 1:250514/1‑80 (MQ=255)
ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt > 2:250523/1‑81 (MQ=255)
ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt > 2:250528/1‑81 (MQ=255)
|
GCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAT > NC_000913/1411036‑1411182
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
