Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,411,109 |
A→T |
D280E (GAT→GAA) |
ttcA ← |
tRNA cytosine(32) 2‑sulfurtransferase TtcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,411,109 | 0 | A | T | 100.0%
| 120.8
/ NA
| 34 | D280E (GAT→GAA) | ttcA | tRNA cytosine(32) 2‑sulfurtransferase TtcA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (22/12); total (22/12) |
CAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCG > NC_000913/1411034‑1411184
|
cAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCaaa > 2:270733/1‑81 (MQ=255)
cAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCaa < 1:270706/80‑1 (MQ=255)
aGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAAc > 2:270743/1‑81 (MQ=255)
cGTAACTCATCCAACTGATTTCCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGcc < 2:270707/81‑1 (MQ=255)
aaCTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc > 1:270765/1‑77 (MQ=255)
cTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGAt > 2:270750/1‑80 (MQ=255)
cATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc > 2:270745/1‑81 (MQ=255)
aTCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcc > 2:270730/1‑81 (MQ=255)
aTCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc < 2:270708/80‑1 (MQ=255)
cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAccccc > 2:270748/1‑81 (MQ=255)
cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcccc < 2:270709/80‑1 (MQ=255)
aCTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGt > 2:270760/1‑81 (MQ=255)
aTCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTc < 2:270710/81‑1 (MQ=255)
ttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAcc > 2:270756/1‑81 (MQ=255)
ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAcc < 2:270711/79‑1 (MQ=255)
ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt > 2:270762/1‑81 (MQ=255)
ggCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg > 2:270764/1‑81 (MQ=255)
gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt < 1:270712/78‑1 (MQ=255)
gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTggg < 1:270713/81‑1 (MQ=255)
cTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGt > 1:270758/1‑81 (MQ=255)
ccAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGAt < 1:270714/81‑1 (MQ=255)
gCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGcc > 2:270753/1‑81 (MQ=255)
aCGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCtt < 2:270715/81‑1 (MQ=255)
aCGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCtt > 2:270751/1‑81 (MQ=255)
cGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGa > 2:270757/1‑80 (MQ=255)
tGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATc > 1:270761/1‑80 (MQ=255)
gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg > 1:270746/1‑80 (MQ=255)
gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg > 2:270754/1‑80 (MQ=255)
aGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa > 1:270744/1‑80 (MQ=255)
tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg < 2:270716/81‑1 (MQ=255)
tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg > 2:270763/1‑81 (MQ=255)
ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt > 2:270759/1‑81 (MQ=255)
ttCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTgg < 1:270717/77‑1 (MQ=255)
tCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCg > 1:270752/1‑81 (MQ=255)
|
CAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCG > NC_000913/1411034‑1411184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
