Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	524	31.4	1748232	97.8%	1709770	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,952	G→A	S100S (TCC→TCT)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,952	0	G	A	100.0%	119.9 / NA	34	S100S (TCC→TCT)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/20); total (14/20)

TCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612878‑1613028

tCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAAtt                                                                         <  1:281567/80‑1 (MQ=255)

   cAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAAc                                                                     <  2:281568/81‑1 (MQ=255)

       ccaccaGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTg                                                                 <  2:281569/81‑1 (MQ=255)

        caccaGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGa                                                                <  1:281570/81‑1 (MQ=255)

             gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg                                                           <  1:281571/81‑1 (MQ=255)

             gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg                                                           <  1:281572/81‑1 (MQ=255)

               gcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGAc                                                         >  1:281607/1‑81 (MQ=255)

                 aCCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACgg                                                       <  1:281574/81‑1 (MQ=255)

                 aCCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACgg                                                       <  1:281573/81‑1 (MQ=255)

                  ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa                                                      <  2:281575/81‑1 (MQ=255)

                  ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa                                                      >  2:281604/1‑81 (MQ=255)

                   cGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGAt                                                     <  1:281576/81‑1 (MQ=255)

                    ggCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATc                                                    <  1:281577/81‑1 (MQ=255)

                         tAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGc                                               >  2:281621/1‑81 (MQ=255)

                              aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg                                          <  2:281578/81‑1 (MQ=255)

                                cGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcgcg                                        >  2:281619/1‑81 (MQ=255)

                                  gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt                                      <  1:281579/81‑1 (MQ=255)

                                        gcgGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGa                                <  2:281580/81‑1 (MQ=255)

                                              taCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTcc                           <  1:281581/80‑1 (MQ=255)

                                              taCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCct                          >  2:281614/1‑81 (MQ=255)

                                               aCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctc                         >  2:281628/1‑81 (MQ=255)

                                                cccTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctct                        <  1:281582/81‑1 (MQ=255)

                                                  cTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTc                      <  2:281583/81‑1 (MQ=255)

                                                   tGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  2:281615/1‑81 (MQ=255)

                                                    gCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  2:574504/1‑80 (MQ=255)

                                                     cTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  1:281624/1‑79 (MQ=255)

                                                       ccATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg                  >  1:281631/1‑80 (MQ=255)

                                                        cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg                  <  1:281585/79‑1 (MQ=255)

                                                        cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGt                 >  2:281618/1‑80 (MQ=255)

                                                        cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGt                 <  1:281584/80‑1 (MQ=255)

                                                         aTTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTaa               >  1:281610/1‑81 (MQ=255)

                                                            tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGa            >  2:281611/1‑81 (MQ=255)

                                                              cAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACg          <  2:281586/81‑1 (MQ=255)

                                                                       tAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGa  >  2:281612/1‑80 (MQ=255)

TCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612878‑1613028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A