Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,612,952 |
G→A |
S100S (TCC→TCT) |
glsB ← |
glutaminase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,612,952 | 0 | G | A | 100.0%
| 109.8
/ NA
| 30 | S100S (TCC→TCT) | glsB | glutaminase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/13); total (17/13) |
CGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA > NC_000913/1612879‑1613028
|
cGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTg > 2:302374/1‑80 (MQ=255)
cAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAAc < 2:302340/81‑1 (MQ=255)
gACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACgg > 1:302369/1‑81 (MQ=255)
accaccAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGt > 1:302379/1‑81 (MQ=255)
accaGCGCACCGGCATTAATGAACGGATT‑CGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATc > 2:302385/1‑81 (MQ=255)
aGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCa < 1:302341/81‑1 (MQ=255)
gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg < 1:302342/81‑1 (MQ=255)
ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa < 2:302343/81‑1 (MQ=255)
ttAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTg < 2:302345/81‑1 (MQ=255)
ttAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTg < 1:302344/81‑1 (MQ=255)
aaTGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGAAGGATCTTTGcc > 2:302386/1‑81 (MQ=255)
aTGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGcc < 1:302346/80‑1 (MQ=255)
gAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAc > 2:302384/1‑81 (MQ=255)
aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTTAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg < 1:302347/81‑1 (MQ=255)
aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg > 2:302382/1‑81 (MQ=255)
aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg > 2:302375/1‑81 (MQ=255)
gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt > 1:302388/1‑81 (MQ=255)
gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt > 1:302387/1‑81 (MQ=255)
aTTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTg > 1:302377/1‑81 (MQ=255)
tACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGc > 1:302376/1‑80 (MQ=255)
cccTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctct < 1:302348/81‑1 (MQ=255)
gCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg > 1:302389/1‑80 (MQ=255)
cTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg < 1:302349/79‑1 (MQ=255)
tCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg > 1:302392/1‑81 (MQ=255)
tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATg < 1:302350/80‑1 (MQ=255)
tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGa > 2:302383/1‑81 (MQ=255)
cAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACg > 2:302399/1‑81 (MQ=255)
ttGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCAt < 1:302352/81‑1 (MQ=255)
ttGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCAt < 1:302351/81‑1 (MQ=255)
tAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGa > 2:302391/1‑80 (MQ=255)
|
CGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA > NC_000913/1612879‑1613028
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
