Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,660,717 |
G→A |
A54A (GCG→GCA) |
ynfF → |
putative selenate reductase YnfF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,660,717 | 0 | G | A | 97.4%
| 130.8
/ ‑4.0
| 39 | A54A (GCG→GCA) | ynfF | putative selenate reductase YnfF |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (22/16); total (23/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
GCTGGCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCGGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTACT > NC_000913/1660642‑1660793
|
gcTGGCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTg > 2:287810/1‑81 (MQ=255)
gcTGGCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTg < 2:287751/81‑1 (MQ=255)
tGGCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTg > 1:287788/1‑79 (MQ=255)
gCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGt > 1:287804/1‑81 (MQ=255)
aGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTg > 1:287805/1‑80 (MQ=255)
cACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCGGTCTGGAGTTCCTGCACCGTTaa > 2:287814/1‑81 (MQ=255)
ctctGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACt < 1:287752/81‑1 (MQ=255)
ctGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGc > 1:287809/1‑81 (MQ=255)
tGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTg < 2:287753/79‑1 (MQ=255)
ccATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCggg < 2:287754/81‑1 (MQ=255)
aTTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAg < 1:287755/81‑1 (MQ=255)
cccAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGc < 1:287756/80‑1 (MQ=255)
ccAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctg < 1:287757/81‑1 (MQ=255)
ccAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctg < 1:287758/81‑1 (MQ=255)
cAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgt > 1:287799/1‑81 (MQ=255)
aaaTGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtc > 1:287800/1‑81 (MQ=255)
aaaTGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtc < 1:287759/81‑1 (MQ=255)
aaTGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtct > 1:287794/1‑81 (MQ=255)
aaTGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtct < 2:287760/81‑1 (MQ=255)
aTGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtctg > 1:287823/1‑81 (MQ=255)
tGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtctgt > 2:287815/1‑81 (MQ=255)
ggTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGctgtctgt > 1:287802/1‑80 (MQ=255)
gTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTa > 2:287798/1‑81 (MQ=255)
cGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGt > 2:287791/1‑81 (MQ=255)
ggcggcAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGttt > 2:287820/1‑81 (MQ=255)
cggcAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTg > 2:287806/1‑80 (MQ=255)
gcAGAGGCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCAt < 2:287761/81‑1 (MQ=255)
ggCTCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGa > 2:287796/1‑80 (MQ=255)
tCCGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGa > 1:287797/1‑77 (MQ=255)
cGGTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGAt > 2:287807/1‑80 (MQ=255)
gTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGac < 1:287762/81‑1 (MQ=255)
gTAGAAGAGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGa > 1:287825/1‑80 (MQ=255)
agaagaGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGacac > 1:287817/1‑81 (MQ=255)
agaagaGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCCGTCTGTTACGTTTGCATGTGAAAGATGacac > 2:287816/1‑81 (MQ=255)
gaagaGAAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACAcc < 1:287763/81‑1 (MQ=255)
agaAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCgtgt < 1:287764/81‑1 (MQ=255)
gaAAGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTa > 1:287803/1‑81 (MQ=255)
aaGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTACt < 1:397754/81‑1 (MQ=255)
aaGCAGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTACt < 1:287765/81‑1 (MQ=255)
|
GCTGGCAAGCAGTGCTTTCACTCTGCCATTTTCCCAAATGGTCCGGGCGGCAGAGGCTCCGGTAGAAGAGAAAGCGGTCTGGAGTTCCTGCACCGTTAACTGCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTACT > NC_000913/1660642‑1660793
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
