Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,806,996 |
G→A |
A209A (GCG→GCA) |
pfkB → |
6‑phosphofructokinase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,806,996 | 0 | G | A | 100.0%
| 108.1
/ NA
| 31 | A209A (GCG→GCA) | pfkB | 6‑phosphofructokinase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/9); total (22/9) |
GTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGA > NC_000913/1806919‑1807070
|
gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAg < 2:309921/81‑1 (MQ=255)
cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCg > 2:309954/1‑81 (MQ=255)
aaCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGt > 1:309957/1‑80 (MQ=255)
ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTaa < 1:309922/81‑1 (MQ=255)
aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGc > 1:309949/1‑81 (MQ=255)
aaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCg < 1:309923/81‑1 (MQ=255)
aCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGc > 1:309945/1‑79 (MQ=255)
cAGTTCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAgcc > 2:309952/1‑79 (MQ=255)
tGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCaa < 1:309924/81‑1 (MQ=255)
gcgcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCaaa > 2:309975/1‑81 (MQ=255)
gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACg > 2:309956/1‑81 (MQ=255)
gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACg > 2:309955/1‑81 (MQ=255)
cTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACgg > 2:309972/1‑81 (MQ=255)
tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACggg > 2:309965/1‑81 (MQ=255)
gAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTcc > 1:309953/1‑81 (MQ=255)
aaCTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTccc > 2:309960/1‑81 (MQ=255)
ccAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTc > 1:309966/1‑80 (MQ=255)
aGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCca > 2:309963/1‑80 (MQ=255)
ggACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg > 1:309964/1‑80 (MQ=255)
aCGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAg < 2:309925/81‑1 (MQ=255)
gATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcg > 1:309980/1‑81 (MQ=255)
aTGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcgc > 1:309950/1‑81 (MQ=255)
tGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCt < 2:309926/81‑1 (MQ=255)
cGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgt < 2:309927/81‑1 (MQ=255)
gCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtg > 2:309969/1‑81 (MQ=255)
cAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt > 2:309977/1‑81 (MQ=255)
aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt > 2:309968/1‑80 (MQ=255)
aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt > 2:309983/1‑80 (MQ=255)
aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTg < 2:309928/81‑1 (MQ=255)
aGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa < 2:309929/81‑1 (MQ=255)
ccGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa > 1:309973/1‑79 (MQ=255)
|
GTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGA > NC_000913/1806919‑1807070
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
