Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,990,813 |
T→A |
V378E (GTG→GAG) |
tyrP → |
tyrosine:H(+) symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,990,813 | 0 | T | A | 96.3%
| 85.5
/ NA
| 27 | V378E (GTG→GAG) | tyrP | tyrosine:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (17/9); minor base G (0/1); total (17/10) |
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTG > NC_000913/1990742‑1990886
|
gTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGtt > 2:339608/1‑81 (MQ=255)
gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTtc < 1:339585/80‑1 (MQ=255)
ccTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTctct > 2:339611/1‑81 (MQ=255)
ggCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCtgtg > 2:339617/1‑81 (MQ=255)
gCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCtgt < 2:339586/79‑1 (MQ=255)
aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTAt < 1:339587/81‑1 (MQ=255)
cAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGc > 2:339628/1‑81 (MQ=255)
aGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCt > 1:339630/1‑81 (MQ=255)
caATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTgg < 1:339588/81‑1 (MQ=255)
aaTCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGc > 2:339619/1‑81 (MQ=255)
aaTCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGc > 1:339624/1‑81 (MQ=255)
ccTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCTGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCTTg > 2:339618/1‑81 (MQ=255)
ggCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAtt > 1:339646/1‑81 (MQ=255)
gCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAttt < 1:339589/81‑1 (MQ=255)
aCCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTg > 1:339626/1‑81 (MQ=255)
ccGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc < 2:339591/81‑1 (MQ=255)
ccGGGTCAAAGGTGGTCGTCCGGCGCTGGAGATGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc < 2:339590/81‑1 (MQ=255)
cAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAgg > 2:339635/1‑81 (MQ=255)
ggtggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttg > 1:339632/1‑81 (MQ=255)
tggtCGTCCGGCGCTGGGGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt < 1:339592/81‑1 (MQ=255)
tggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt < 2:339593/81‑1 (MQ=255)
tggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt < 2:339594/81‑1 (MQ=255)
gtcgtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAc > 2:339627/1‑81 (MQ=255)
gtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAc > 1:339629/1‑78 (MQ=255)
ccGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAg > 1:339636/1‑79 (MQ=255)
ccGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAg > 1:339623/1‑79 (MQ=255)
gcgcTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTg > 1:339631/1‑81 (MQ=255)
|
GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTG > NC_000913/1990742‑1990886
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
