Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,222,467 |
G→A |
D95N (GAT→AAT) |
dld → |
D‑lactate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,222,467 | 0 | G | A | 100.0%
| 136.6
/ NA
| 39 | D95N (GAT→AAT) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/12); total (27/12) |
ACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAT > NC_000913/2222390‑2222541
|
aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg > 1:377927/1‑81 (MQ=255)
cAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGt < 1:377891/81‑1 (MQ=255)
cAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGt < 2:377890/81‑1 (MQ=255)
aaaaTTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTc < 2:377892/81‑1 (MQ=255)
attattCTGATGCAGGCCTCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGt > 1:377921/1‑80 (MQ=255)
tattCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTat > 2:377940/1‑81 (MQ=255)
ttCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatc < 1:377893/80‑1 (MQ=255)
tGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTAtcatca > 1:377924/1‑81 (MQ=255)
ggCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCt > 2:377929/1‑81 (MQ=255)
ggCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCt < 2:377894/81‑1 (MQ=255)
ccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGc > 1:377926/1‑81 (MQ=255)
cgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCg > 2:377930/1‑81 (MQ=255)
aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc > 2:377939/1‑81 (MQ=255)
aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGa > 1:377935/1‑80 (MQ=255)
aGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGAc > 2:377944/1‑79 (MQ=255)
ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg > 1:377957/1‑81 (MQ=255)
gCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCATCCTGCGTCTCGACAAGc > 1:377925/1‑81 (MQ=255)
cTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTg < 2:377895/81‑1 (MQ=255)
gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa > 2:377941/1‑81 (MQ=255)
gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa > 2:377931/1‑81 (MQ=255)
gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa > 1:377954/1‑81 (MQ=255)
gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt > 1:377959/1‑81 (MQ=255)
gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt > 2:377937/1‑81 (MQ=255)
ggATCGACGCCAAACGGTAACCATTATTATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCtt > 2:377928/1‑81 (MQ=255)
gATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTg < 2:377896/81‑1 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTgg > 1:377976/1‑80 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTgg > 1:377961/1‑80 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc < 1:377897/81‑1 (MQ=255)
aaaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCg > 1:377950/1‑80 (MQ=255)
aaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 2:377933/1‑81 (MQ=255)
ggTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg > 2:377952/1‑81 (MQ=255)
ggTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg > 2:377934/1‑81 (MQ=255)
tAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGt > 2:377938/1‑81 (MQ=255)
cGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt < 1:377898/81‑1 (MQ=255)
cGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt > 1:377949/1‑81 (MQ=255)
aTTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTgg < 1:377899/81‑1 (MQ=255)
ttATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGc < 2:377900/81‑1 (MQ=255)
aTGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGcc > 2:377948/1‑80 (MQ=255)
aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt < 2:377901/80‑1 (MQ=255)
|
ACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAT > NC_000913/2222390‑2222541
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
