Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,222,467 |
G→A |
D95N (GAT→AAT) |
dld → |
D‑lactate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,222,467 | 0 | G | A | 100.0%
| 187.4
/ NA
| 50 | D95N (GAT→AAT) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/23); total (27/23) |
GACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATC > NC_000913/2222389‑2222542
|
gACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAAt > 2:404029/1‑81 (MQ=255)
aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg > 1:404044/1‑81 (MQ=255)
aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg > 2:404032/1‑81 (MQ=255)
aaTTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGt < 1:403983/81‑1 (MQ=255)
attattCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGtt < 2:403984/81‑1 (MQ=255)
attCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTat < 1:403985/80‑1 (MQ=255)
ttCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatca < 2:403986/81‑1 (MQ=255)
cTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatcat < 1:403988/80‑1 (MQ=255)
cTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatcat < 1:403987/80‑1 (MQ=255)
aTGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAg < 2:403989/80‑1 (MQ=255)
gCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAc > 1:404037/1‑81 (MQ=255)
cAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAcc < 1:403990/81‑1 (MQ=255)
cAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAcc > 2:404033/1‑81 (MQ=255)
aGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCAccc > 2:404049/1‑81 (MQ=255)
gccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTg < 2:403991/81‑1 (MQ=255)
ccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGc > 2:404046/1‑81 (MQ=255)
cgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCg < 1:403992/81‑1 (MQ=255)
ccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtc < 2:403993/81‑1 (MQ=255)
aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc > 2:404030/1‑81 (MQ=255)
aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc > 1:404028/1‑81 (MQ=255)
aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc < 1:403994/81‑1 (MQ=255)
aTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc > 2:404041/1‑80 (MQ=255)
ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg > 1:404054/1‑81 (MQ=255)
ggCCTAACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGCCGTTATCATCAGCACCCTGCGTCTCGACAAg > 2:404067/1‑81 (MQ=255)
gCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGc > 2:404045/1‑81 (MQ=255)
aCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGc < 2:403996/79‑1 (MQ=255)
aCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGc < 2:403995/79‑1 (MQ=255)
cGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGt > 2:404061/1‑81 (MQ=255)
gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt > 1:404058/1‑81 (MQ=255)
aaGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc > 2:404042/1‑81 (MQ=255)
aGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCt < 1:403997/81‑1 (MQ=255)
ggATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCtt < 2:403998/81‑1 (MQ=255)
gATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTg > 1:404040/1‑81 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc < 1:403999/81‑1 (MQ=255)
cGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc > 2:404051/1‑80 (MQ=255)
gACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCaa < 2:635631/81‑1 (MQ=255)
aCGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAg < 2:404000/81‑1 (MQ=255)
aCGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAg > 1:404043/1‑81 (MQ=255)
aaaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCg > 1:404038/1‑80 (MQ=255)
cGGTAACGATTATGATTGCAATGTCGTTATCATCAGCACCCTGCGTCCCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 1:404047/1‑79 (MQ=255)
cGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACa < 1:404001/81‑1 (MQ=255)
gTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAgg > 1:404050/1‑81 (MQ=255)
aaCGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTg > 2:404056/1‑81 (MQ=255)
aCGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGc < 1:404002/81‑1 (MQ=255)
gATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTg > 1:404066/1‑81 (MQ=255)
aTGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGcc > 1:404065/1‑80 (MQ=255)
tGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt < 1:404003/80‑1 (MQ=255)
aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc > 1:404052/1‑81 (MQ=255)
aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc < 1:404004/81‑1 (MQ=255)
aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc > 2:404055/1‑81 (MQ=255)
|
GACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATC > NC_000913/2222389‑2222542
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
