Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,234,328 |
G→A |
G99D (GGC→GAC) |
preT → |
NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,234,328 | 0 | G | A | 100.0%
| 67.7
/ NA
| 20 | G99D (GGC→GAC) | preT | NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/9); total (11/9) |
AGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCG > NC_000913/2234255‑2234405
|
agagTGTGCCCGACGGAGAGATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTa > 2:379966/1‑81 (MQ=255)
tgtgCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGc < 2:379946/81‑1 (MQ=255)
tgCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCg < 1:379947/80‑1 (MQ=255)
cccGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGttt < 2:379948/81‑1 (MQ=255)
aCGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGt > 1:379981/1‑80 (MQ=255)
gagaAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACt > 2:379975/1‑81 (MQ=255)
agaAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACt > 2:379969/1‑80 (MQ=255)
ccAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaaca < 1:379949/81‑1 (MQ=255)
cAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaacaa < 2:379950/81‑1 (MQ=255)
gTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCg > 2:379977/1‑81 (MQ=255)
tGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCgg > 1:379984/1‑80 (MQ=255)
cGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGa > 1:379973/1‑80 (MQ=255)
gCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAAtt > 2:379979/1‑81 (MQ=255)
ggTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTAt < 2:379951/81‑1 (MQ=255)
cTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTa < 1:379952/81‑1 (MQ=255)
aTTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTaa > 2:379976/1‑80 (MQ=255)
tatCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCt > 1:379978/1‑81 (MQ=255)
atCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTc > 2:379986/1‑81 (MQ=255)
tCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCg < 2:379954/81‑1 (MQ=255)
tCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCg < 1:379953/81‑1 (MQ=255)
|
AGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCG > NC_000913/2234255‑2234405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
