Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,374,272 |
T→C |
L244L (TTA→TTG) |
menE ← |
o‑succinylbenzoate‑‑CoA ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,374,272 | 0 | T | C | 100.0%
| 122.5
/ NA
| 35 | L244L (TTA→TTG) | menE | o‑succinylbenzoate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/13); total (22/13) |
CATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTG > NC_000913/2374196‑2374346
|
cATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAaca > 1:404796/1‑81 (MQ=255)
aTAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAacac > 1:404794/1‑81 (MQ=255)
aTAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGC‑‑CCAAGCAACACCg > 1:404774/1‑81 (MQ=255)
cGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCtt > 2:404787/1‑81 (MQ=255)
gCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt < 2:404755/81‑1 (MQ=255)
gCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt > 1:404797/1‑81 (MQ=255)
cAAAAGCAACGAATCGCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt > 2:404788/1‑80 (MQ=255)
cAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCttt > 1:404802/1‑80 (MQ=255)
cAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTc < 2:404756/81‑1 (MQ=255)
gCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAggg > 1:404792/1‑80 (MQ=255)
aCGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAAc < 2:404757/81‑1 (MQ=255)
cGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACg > 1:404801/1‑81 (MQ=255)
aTCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGaa < 1:404758/81‑1 (MQ=255)
cccTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTa > 1:404805/1‑81 (MQ=255)
tGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACgg > 1:404800/1‑81 (MQ=255)
gCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGt > 2:404795/1‑81 (MQ=255)
cgcgcgCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTaa > 1:404759/1‑81 (MQ=255)
gcgcgcCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAAc < 1:404760/81‑1 (MQ=255)
gcCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGc < 2:404761/81‑1 (MQ=255)
tGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCaaa > 1:404807/1‑81 (MQ=255)
ttCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACATCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACg > 2:404806/1‑81 (MQ=255)
ccGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGc > 1:404776/1‑80 (MQ=255)
cGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGcc < 2:404762/80‑1 (MQ=255)
cGTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGc > 2:404817/1‑79 (MQ=255)
gTCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCag < 1:404763/81‑1 (MQ=255)
tCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCaga < 2:404765/81‑1 (MQ=255)
tCAACTCGACCGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCaga < 2:404764/81‑1 (MQ=255)
cGGGATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTgg > 1:404813/1‑81 (MQ=255)
gggATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTgg > 2:404814/1‑80 (MQ=255)
ggATAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCa > 2:404810/1‑81 (MQ=255)
aTAGCCGCGCCGCCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCAc > 2:404790/1‑80 (MQ=255)
cgcgccgcCAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGa < 1:404766/81‑1 (MQ=255)
gccgccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGc < 1:404767/81‑1 (MQ=255)
gccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGt > 2:404803/1‑80 (MQ=255)
gccAAGCAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTg < 2:404768/81‑1 (MQ=255)
|
CATAGCCGCAAAAGCAACGAATCCCCTGCTCGCGCGCCTGTTCCGTCAACTCGACCGGGATAGCCGCGCCGCCAAGTAACACCGCTTTCAGGGAAACGGAACTACGGTTAACCAGCAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTG > NC_000913/2374196‑2374346
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
