Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,411,277 |
T→C |
M26V (ATG→GTG) |
yfbS ← |
putative transporter YfbS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,411,277 | 0 | T | C | 100.0%
| 78.9
/ NA
| 23 | M26V (ATG→GTG) | yfbS | putative transporter YfbS |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/9); total (14/9) |
GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA > NC_000913/2411203‑2411351
|
ggAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCAc > 2:411558/1‑81 (MQ=255)
gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCAct < 2:411519/81‑1 (MQ=255)
aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCActc > 1:411547/1‑81 (MQ=255)
aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTg < 1:411520/81‑1 (MQ=255)
cTTCTGTTACTGTCAGCGTTCCGCTTAATTCAAACGCTACAATAACAAACAAATCGACCGCATCCACACTCACTCTTccct > 2:411563/1‑80 (MQ=255)
cTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGt < 1:411522/79‑1 (MQ=255)
cTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGt < 2:411521/79‑1 (MQ=255)
gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAATCGACCGCATCCACACGCACTCTGCCCGTCGc > 2:411559/1‑80 (MQ=255)
aCTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCaaa > 1:411553/1‑80 (MQ=255)
tCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAg < 2:411523/80‑1 (MQ=255)
tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATgg > 1:411555/1‑80 (MQ=255)
aTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCa < 1:411524/81‑1 (MQ=255)
aaCGCGACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt < 2:411525/81‑1 (MQ=255)
gACAATAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa > 1:411560/1‑78 (MQ=255)
aTAACAAACAAAGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc > 1:411556/1‑81 (MQ=255)
aaacaaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt > 1:411562/1‑81 (MQ=255)
acaaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa < 1:411526/81‑1 (MQ=255)
caaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa > 1:411569/1‑81 (MQ=255)
caaaGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa > 2:411554/1‑81 (MQ=255)
aGCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt > 1:411552/1‑80 (MQ=255)
gCGACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa < 1:411527/81‑1 (MQ=255)
gACCGCATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc > 2:411570/1‑81 (MQ=255)
cATCCACACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca > 2:411572/1‑81 (MQ=255)
|
GGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCA > NC_000913/2411203‑2411351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
