Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,436,213 |
T→A |
D146V (GAT→GTT) |
usg ← |
putative semialdehyde dehydrogenase Usg |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,436,213 | 0 | T | A | 100.0%
| 108.3
/ NA
| 31 | D146V (GAT→GTT) | usg | putative semialdehyde dehydrogenase Usg |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (15/16); total (15/16) |
GCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTA > NC_000913/2436138‑2436289
|
gCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGAtt < 2:416117/81‑1 (MQ=255)
cATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGAtt > 2:416167/1‑80 (MQ=255)
gACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACgg < 1:416119/81‑1 (MQ=255)
gACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACgg > 1:416154/1‑81 (MQ=255)
ttttttGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAg > 2:416156/1‑81 (MQ=255)
gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag > 1:416158/1‑79 (MQ=255)
gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag > 1:416162/1‑79 (MQ=255)
gggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcag > 1:416170/1‑79 (MQ=255)
ggCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCcagc < 2:416120/79‑1 (MQ=255)
ggAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAgctg < 2:416121/81‑1 (MQ=255)
gCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAgctggctg < 2:416122/81‑1 (MQ=255)
cTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGctggctgg < 2:416123/81‑1 (MQ=255)
tGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGt > 1:416163/1‑81 (MQ=255)
aaaTCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTc < 1:416124/80‑1 (MQ=255)
aaTCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGt > 2:416178/1‑78 (MQ=255)
tGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTg > 1:416177/1‑81 (MQ=255)
tGGTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTg > 2:416174/1‑81 (MQ=255)
ggTAACGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTgg > 2:416157/1‑81 (MQ=255)
aaCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTAc > 1:416168/1‑81 (MQ=255)
aaCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTAc > 1:416153/1‑81 (MQ=255)
aCGCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACg < 2:416125/81‑1 (MQ=255)
gCTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGc < 1:416126/81‑1 (MQ=255)
cTGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCg < 2:416127/81‑1 (MQ=255)
tGATACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGa > 2:416191/1‑81 (MQ=255)
aTACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCTATg < 2:416128/81‑1 (MQ=255)
aTACGTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATg < 2:416129/81‑1 (MQ=255)
gTGATAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAt > 1:416165/1‑81 (MQ=255)
aTAAACCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttcc < 2:416130/81‑1 (MQ=255)
aCCGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggtt < 2:416131/81‑1 (MQ=255)
ccGCCCTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggttc < 1:416132/81‑1 (MQ=255)
cTGAACGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTa < 1:416133/81‑1 (MQ=255)
|
GCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTA > NC_000913/2436138‑2436289
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
