Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,582,923 |
T→C |
T186A (ACG→GCG) |
nudK ← |
GDP‑mannose hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,582,923 | 0 | T | C | 100.0%
| 66.8
/ NA
| 20 | T186A (ACG→GCG) | nudK | GDP‑mannose hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/10); total (10/10) |
AATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCT > NC_000913/2582848‑2582998
|
aaTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGc > 1:441146/1‑80 (MQ=255)
aTCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCaa < 1:441125/81‑1 (MQ=255)
ggATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATa < 2:441126/81‑1 (MQ=255)
gTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTaa < 1:441127/80‑1 (MQ=255)
tCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAg > 2:441152/1‑80 (MQ=255)
aataatAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTc < 2:441128/81‑1 (MQ=255)
ataaACAACGAAATATTATTGTTATTTTCAAGTCCATTAAAGGTGACGCTTGCAAATAGTTAAGCAATAAACCCGCCTTAc > 1:441154/1‑81 (MQ=255)
aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGt < 2:441129/81‑1 (MQ=255)
acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACg < 2:441130/81‑1 (MQ=255)
cGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACg > 2:441162/1‑80 (MQ=255)
cGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGt < 2:441131/81‑1 (MQ=255)
aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAt < 1:441132/81‑1 (MQ=255)
tattGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGcc > 2:441171/1‑81 (MQ=255)
attGTTATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGcc < 1:441133/80‑1 (MQ=255)
ttATTTTTCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGttt > 2:441153/1‑81 (MQ=255)
ttttCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGat > 2:441150/1‑81 (MQ=255)
tCAGTCCATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGatcat > 1:441155/1‑81 (MQ=255)
ccATTAAATGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCAtctc > 2:441170/1‑79 (MQ=255)
aaTGTGACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCt < 1:441134/81‑1 (MQ=255)
gACGCTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCt > 1:441156/1‑80 (MQ=255)
|
AATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCT > NC_000913/2582848‑2582998
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
