Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,604,683 |
A→G |
S279S (TCA→TCG) |
hyfC → |
hydrogenase 4 component C |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,604,683 | 0 | A | G | 100.0%
| 147.0
/ NA
| 41 | S279S (TCA→TCG) | hyfC | hydrogenase 4 component C |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/23); total (18/23) |
TTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGC > NC_000913/2604611‑2604760
|
ttCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAg > 2:445299/1‑80 (MQ=255)
ctctcGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGaa < 2:445241/80‑1 (MQ=255)
tcGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAaca > 1:445285/1‑81 (MQ=255)
cGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAacac > 2:445293/1‑81 (MQ=255)
ctgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCt < 2:445243/81‑1 (MQ=255)
ctgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCt < 2:445242/81‑1 (MQ=255)
tgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTg > 1:445287/1‑81 (MQ=255)
tgcctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTg > 1:445305/1‑81 (MQ=255)
cctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTgg < 2:445244/80‑1 (MQ=255)
cctgcTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGc < 2:445245/81‑1 (MQ=255)
ctgACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCAcgc < 2:445247/81‑1 (MQ=255)
ctgACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCAc < 2:445246/79‑1 (MQ=255)
cACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGtt > 2:445307/1‑81 (MQ=255)
aCTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGttt > 2:445290/1‑81 (MQ=255)
cTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGtttt < 1:445248/81‑1 (MQ=255)
gTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTa > 1:445302/1‑81 (MQ=255)
gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt > 1:445295/1‑80 (MQ=255)
gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt > 1:445315/1‑80 (MQ=255)
gTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTc < 1:445249/81‑1 (MQ=255)
aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAtt > 1:445296/1‑81 (MQ=255)
aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAtt > 2:445313/1‑81 (MQ=255)
aCGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAt < 1:445250/80‑1 (MQ=255)
cGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTc < 1:445251/81‑1 (MQ=255)
gctCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCa > 1:445292/1‑81 (MQ=255)
tCAAGGTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCAtg > 1:445310/1‑81 (MQ=255)
ggTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc < 1:445252/81‑1 (MQ=255)
ggTTTTGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc < 1:445253/81‑1 (MQ=255)
ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg > 1:445298/1‑81 (MQ=255)
ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg > 1:445286/1‑81 (MQ=255)
ttGCTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtgg > 2:445294/1‑81 (MQ=255)
gCTGATTTTTGTACTGGCCTCGATCGCAGAGAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtggc < 2:445254/80‑1 (MQ=255)
cTGATTTTTGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtggct < 2:445255/80‑1 (MQ=255)
ttGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTc < 1:445256/80‑1 (MQ=255)
tGTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCa < 2:445257/80‑1 (MQ=255)
gTACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGc > 1:445303/1‑81 (MQ=255)
tACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGcc < 2:445258/81‑1 (MQ=255)
tACTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGc < 2:445259/80‑1 (MQ=255)
cTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCtt < 2:445260/81‑1 (MQ=255)
cTGGCCTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCt < 2:445261/80‑1 (MQ=255)
ccTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtgctg < 2:445262/81‑1 (MQ=255)
cTCGATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtgctgc < 2:445263/81‑1 (MQ=255)
|
TTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGC > NC_000913/2604611‑2604760
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
