Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,659,676 |
A→T |
F71I (TTT→ATT) |
iscA ← |
iron‑sulfur cluster insertion protein IscA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,659,676 | 0 | A | T | 100.0%
| 81.0
/ NA
| 24 | F71I (TTT→ATT) | iscA | iron‑sulfur cluster insertion protein IscA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (15/9); total (15/9) |
TTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTT > NC_000913/2659600‑2659747
|
ttGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTg > 1:455914/1‑80 (MQ=255)
gACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAg > 2:455916/1‑81 (MQ=255)
aCGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAgg > 2:455912/1‑81 (MQ=255)
cGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGc > 1:455915/1‑81 (MQ=255)
gTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGct > 1:455920/1‑81 (MQ=255)
cGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCtt > 2:455917/1‑81 (MQ=255)
ggTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGc > 1:455927/1‑81 (MQ=255)
gTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGc > 2:455919/1‑80 (MQ=255)
aaTTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGAc < 2:455888/80‑1 (MQ=255)
tttAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCAc > 2:455924/1‑81 (MQ=255)
tAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACg < 2:455889/80‑1 (MQ=255)
tAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACg < 2:455890/80‑1 (MQ=255)
aCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACtt < 2:455891/81‑1 (MQ=255)
cccTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACttt > 1:455922/1‑81 (MQ=255)
cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGTCCTTGCCATCGACCACGACTTTCACGCCttt > 2:455931/1‑81 (MQ=255)
cttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTc < 2:455892/79‑1 (MQ=255)
ttACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAac < 1:455893/80‑1 (MQ=255)
aaGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATg < 2:455894/81‑1 (MQ=255)
ccAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCtt > 2:455930/1‑81 (MQ=255)
cAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTc < 2:455895/81‑1 (MQ=255)
gCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCg > 2:455933/1‑81 (MQ=255)
ccGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGt < 1:455896/81‑1 (MQ=255)
cGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGtt > 1:455926/1‑81 (MQ=255)
cGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGtt > 2:455938/1‑81 (MQ=255)
|
TTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTT > NC_000913/2659600‑2659747
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
