Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,659,676 |
A→T |
F71I (TTT→ATT) |
iscA ← |
iron‑sulfur cluster insertion protein IscA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,659,676 | 0 | A | T | 100.0%
| 132.3
/ NA
| 37 | F71I (TTT→ATT) | iscA | iron‑sulfur cluster insertion protein IscA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/20); total (17/20) |
GTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTC > NC_000913/2659605‑2659751
|
gTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGct < 2:487457/81‑1 (MQ=255)
gTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGct > 1:487508/1‑81 (MQ=255)
ggTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGc > 2:487527/1‑81 (MQ=255)
aTTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCa < 1:487458/81‑1 (MQ=255)
tttAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCAc < 1:487459/81‑1 (MQ=255)
aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGAc > 1:487510/1‑81 (MQ=255)
aaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGAc < 2:487460/80‑1 (MQ=255)
aaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACt > 2:487509/1‑81 (MQ=255)
aCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACtt < 2:487461/81‑1 (MQ=255)
gTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGcc > 2:487506/1‑81 (MQ=255)
tCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt > 1:487504/1‑81 (MQ=255)
cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCttt > 1:487516/1‑81 (MQ=255)
cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt < 2:487463/80‑1 (MQ=255)
cAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCtt < 2:487462/80‑1 (MQ=255)
aGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCttt < 2:487464/80‑1 (MQ=255)
ccTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCt > 1:487523/1‑81 (MQ=255)
cttcttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCtt > 2:487507/1‑81 (MQ=255)
cttcttTTACGAAGTCCAGCTGCGTACCGTCCAGAATCTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCtt < 1:487465/81‑1 (MQ=255)
cttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCa < 2:487466/80‑1 (MQ=255)
cttTTACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCa > 2:487514/1‑80 (MQ=255)
ttttACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCaaa > 1:487512/1‑81 (MQ=255)
ttttACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCaaa > 1:487511/1‑81 (MQ=255)
tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAacac > 1:487546/1‑81 (MQ=255)
tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca < 1:487468/80‑1 (MQ=255)
tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca < 1:487467/80‑1 (MQ=255)
tACGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca < 2:487469/80‑1 (MQ=255)
aCGAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAacac < 2:487470/80‑1 (MQ=255)
gAAGTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGAt > 2:487519/1‑81 (MQ=255)
gTCCAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTc < 2:487471/81‑1 (MQ=255)
cAGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCtt < 2:487472/80‑1 (MQ=255)
aGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc < 1:487473/81‑1 (MQ=255)
aGCTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc < 1:487474/81‑1 (MQ=255)
cTGCGTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCgg < 1:487475/81‑1 (MQ=255)
gTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTc < 1:487476/81‑1 (MQ=255)
gTACCGTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTc > 1:487513/1‑81 (MQ=255)
gTCCAGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTc > 2:487526/1‑81 (MQ=255)
aGAATTTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTc > 2:487520/1‑80 (MQ=255)
|
GTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTC > NC_000913/2659605‑2659751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
