Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,899,373 |
C→T |
V16I (GTT→ATT) |
ygcU ← |
putative FAD‑containing dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,899,373 | 0 | C | T | 100.0%
| 70.0
/ NA
| 21 | V16I (GTT→ATT) | ygcU | putative FAD‑containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/12); total (9/12) |
AATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGA > NC_000913/2899303‑2899447
|
aaTTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCttt < 1:500765/81‑1 (MQ=255)
tACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCt > 2:500793/1‑81 (MQ=255)
cGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTgg < 1:500766/81‑1 (MQ=255)
aCGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGa > 1:500785/1‑80 (MQ=255)
cGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACa < 2:500767/81‑1 (MQ=255)
ggTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACaa > 1:500787/1‑81 (MQ=255)
ggTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACaa > 1:500797/1‑81 (MQ=255)
tCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATc < 1:500768/81‑1 (MQ=255)
tttCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCgag < 2:500769/80‑1 (MQ=255)
tCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGAt < 2:500770/80‑1 (MQ=255)
tAATACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAg < 1:500771/80‑1 (MQ=255)
tACTGTTTCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACAt < 1:500772/81‑1 (MQ=255)
tttCATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTaa > 2:500794/1‑81 (MQ=255)
cATCGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttc > 2:500804/1‑81 (MQ=255)
cGGTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttcctt > 2:500796/1‑81 (MQ=255)
ggTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttccttc > 2:500792/1‑81 (MQ=255)
ggTAATTACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAAttcctt < 1:500773/80‑1 (MQ=255)
ttACGCGATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTTat < 2:500774/81‑1 (MQ=255)
gcgATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGataata < 1:500775/81‑1 (MQ=255)
gcgATCTGCACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGataat > 1:500800/1‑80 (MQ=255)
cACCAATAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGa < 2:500776/81‑1 (MQ=255)
|
AATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTCGACAATCGCTGCGCGAGATAAAGACATAGTAATTCCTTCCTGATAATAAAAATGGA > NC_000913/2899303‑2899447
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
