Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,900,805 |
T→A |
F72I (TTT→ATT) |
yqcE → |
putative transport protein YqcE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,900,805 | 0 | T | A | 100.0%
| 62.8
/ NA
| 18 | F72I (TTT→ATT) | yqcE | putative transport protein YqcE |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/8); total (10/8) |
GTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAATTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCG > NC_000913/2900734‑2900882
|
gTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACAcc < 1:536645/81‑1 (MQ=255)
aataatGAGTACCTTTGGTGTTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCa < 1:536646/80‑1 (MQ=255)
aataatGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCa > 1:536676/1‑80 (MQ=255)
gCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCgatgat < 2:536647/80‑1 (MQ=255)
gCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCAtt < 1:536648/81‑1 (MQ=255)
ccATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTa < 2:536649/81‑1 (MQ=255)
attattCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTAcc > 1:536672/1‑81 (MQ=255)
ttATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACt > 2:536680/1‑81 (MQ=255)
gCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGt > 1:536683/1‑81 (MQ=255)
cccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTc < 2:536650/81‑1 (MQ=255)
ccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCt > 1:536675/1‑81 (MQ=255)
ccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCt > 2:536674/1‑81 (MQ=255)
aGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTa < 1:536651/81‑1 (MQ=255)
gcggcgTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTaa > 1:536685/1‑81 (MQ=255)
gcgTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATgg > 2:536700/1‑81 (MQ=255)
aTTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACg > 1:536691/1‑81 (MQ=255)
gCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTAt > 1:536693/1‑81 (MQ=255)
ccGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATc < 1:536652/81‑1 (MQ=255)
aaaaTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCg > 1:536690/1‑81 (MQ=255)
|
GTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAATTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCG > NC_000913/2900734‑2900882
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
