Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,906,427 |
A→G |
I239M (ATA→ATG) |
ygcG → |
protein YgcG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,906,427 | 0 | A | G | 100.0%
| 62.3
/ NA
| 18 | I239M (ATA→ATG) | ygcG | protein YgcG |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/8); total (10/8) |
TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAAC > NC_000913/2906352‑2906496
|
tctTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGttt < 2:537577/79‑1 (MQ=255)
ttATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTAcc > 1:537597/1‑81 (MQ=255)
tATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTAcc < 2:537578/80‑1 (MQ=255)
tCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCGCCATGTTTATGGTCTTTACCTGtt > 2:537602/1‑81 (MQ=255)
ccTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGtt < 2:537579/80‑1 (MQ=255)
ttCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTAtgt > 1:537601/1‑81 (MQ=255)
ttCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTAt < 1:537580/79‑1 (MQ=255)
aTAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACtt > 1:537599/1‑80 (MQ=255)
aaaaTTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACtt > 1:537604/1‑78 (MQ=255)
ccATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCAt > 1:537608/1‑81 (MQ=255)
aTTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCt > 2:537606/1‑81 (MQ=255)
aTTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAg > 2:537603/1‑81 (MQ=255)
tttCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGt > 1:537609/1‑80 (MQ=255)
tCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTa < 1:537581/80‑1 (MQ=255)
tCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTAt > 1:537605/1‑81 (MQ=255)
cttTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCAt < 2:537582/79‑1 (MQ=255)
aCTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaa < 1:537583/80‑1 (MQ=255)
cTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac < 2:537584/80‑1 (MQ=255)
|
TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAAC > NC_000913/2906352‑2906496
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
