Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,907,489 |
C→T |
M151I (ATG→ATA) |
eno ← |
enolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,907,489 | 0 | C | T | 100.0%
| 60.6
/ NA
| 18 | M151I (ATG→ATA) | eno | enolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/11); total (7/11) |
TTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCA > NC_000913/2907412‑2907559
|
ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttat > 2:537789/1‑80 (MQ=255)
cgcgCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTtatcat > 1:537806/1‑81 (MQ=255)
gcgcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTatcatc > 2:537791/1‑81 (MQ=255)
gcgcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTatcatc < 2:537750/81‑1 (MQ=255)
cAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGaa < 2:537751/81‑1 (MQ=255)
aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAAc > 2:537783/1‑81 (MQ=255)
gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa < 1:537752/81‑1 (MQ=255)
tGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 1:537793/1‑79 (MQ=255)
tGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 1:537792/1‑79 (MQ=255)
tGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTAtt > 1:537794/1‑81 (MQ=255)
cTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCgg < 2:537753/81‑1 (MQ=255)
atCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAcc > 1:537800/1‑81 (MQ=255)
aaCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt < 2:537754/81‑1 (MQ=255)
tGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGc < 1:537755/81‑1 (MQ=255)
cTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTc < 2:537756/81‑1 (MQ=255)
accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGt < 1:537757/80‑1 (MQ=255)
ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc < 2:537758/81‑1 (MQ=255)
caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa > 1:537796/1‑79 (MQ=255)
caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACa < 2:537759/81‑1 (MQ=255)
gatgatGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa < 2:537760/80‑1 (MQ=255)
|
TTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCA > NC_000913/2907412‑2907559
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
