Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,907,489 |
C→T |
M151I (ATG→ATA) |
eno ← |
enolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,907,489 | 0 | C | T | 100.0%
| 95.8
/ NA
| 28 | M151I (ATG→ATA) | eno | enolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/8); total (20/8) |
TTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCG > NC_000913/2907411‑2907556
|
tttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttat > 2:501905/1‑81 (MQ=255)
cgcgCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttatca < 1:501863/80‑1 (MQ=255)
ggCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGc < 1:501864/81‑1 (MQ=255)
gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 2:501900/1‑81 (MQ=255)
gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 1:501899/1‑81 (MQ=255)
cTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCAt < 1:501865/81‑1 (MQ=255)
tGAGTCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 2:501891/1‑79 (MQ=255)
gAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa > 1:501903/1‑78 (MQ=255)
aaTCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATag < 1:501866/80‑1 (MQ=255)
tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATagag > 1:501906/1‑80 (MQ=255)
gAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTAttt > 2:501918/1‑81 (MQ=255)
aTTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGc < 1:501867/81‑1 (MQ=255)
gATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGt < 1:501868/81‑1 (MQ=255)
cAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgt > 1:501907/1‑81 (MQ=255)
aCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttc < 2:501869/81‑1 (MQ=255)
aCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt > 1:501911/1‑80 (MQ=255)
ttgttgTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagt > 1:501924/1‑81 (MQ=255)
tgttgtCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagtt > 1:501922/1‑81 (MQ=255)
tCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGc > 2:501910/1‑80 (MQ=255)
tGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGc > 2:501914/1‑81 (MQ=255)
gCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCt > 2:501909/1‑81 (MQ=255)
caccacCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg > 2:501923/1‑80 (MQ=255)
accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg < 2:501870/79‑1 (MQ=255)
accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa > 1:501920/1‑81 (MQ=255)
ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa > 1:501919/1‑80 (MQ=255)
ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc < 2:501871/81‑1 (MQ=255)
caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa > 2:501913/1‑79 (MQ=255)
caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa > 2:501965/1‑79 (MQ=255)
accGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAg > 1:501901/1‑81 (MQ=255)
cGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCg > 1:501925/1‑81 (MQ=255)
|
TTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCG > NC_000913/2907411‑2907556
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
