Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,008,057 |
G→A |
G183D (GGC→GAC) |
ygeX → |
2,3‑diaminopropionate ammonia‑lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,008,057 | 0 | G | A | 100.0%
| 153.9
/ NA
| 44 | G183D (GGC→GAC) | ygeX | 2,3‑diaminopropionate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/20); total (24/20) |
CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCC > NC_000913/3007980‑3008134
|
cGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTg > 2:520346/1‑81 (MQ=255)
ggTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACt < 2:520305/79‑1 (MQ=255)
ggTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTgg > 2:520359/1‑81 (MQ=255)
tGCCGAGTGCAACGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGa < 2:520306/81‑1 (MQ=255)
gCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTggg > 1:520348/1‑79 (MQ=255)
tGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa < 2:520307/81‑1 (MQ=255)
gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa > 2:520356/1‑80 (MQ=255)
gCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTa > 1:520385/1‑80 (MQ=255)
aTCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAg < 2:520308/81‑1 (MQ=255)
cGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGa > 2:520366/1‑81 (MQ=255)
aCGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACg > 2:520353/1‑81 (MQ=255)
cGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACgg > 2:520370/1‑81 (MQ=255)
ggATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGc < 1:520309/81‑1 (MQ=255)
gATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCa > 2:520354/1‑81 (MQ=255)
aaCTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGa < 2:520310/80‑1 (MQ=255)
cTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAgg < 2:520311/81‑1 (MQ=255)
tATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt > 2:520372/1‑81 (MQ=255)
aTGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt > 1:520360/1‑80 (MQ=255)
tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGt < 1:520313/79‑1 (MQ=255)
tGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTa < 2:520312/81‑1 (MQ=255)
aCGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTac < 2:520314/80‑1 (MQ=255)
cGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTacac < 1:520315/81‑1 (MQ=255)
gATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACAcc < 2:520316/81‑1 (MQ=255)
aTACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACAcc > 2:520387/1‑80 (MQ=255)
aTACCGTTCGCCTGACCATGCAACACCCGCAGCAGCACGACCGGGAAGTGGTCCAGCACACGGCCTGGGAAGGTCACAcc > 2:520378/1‑80 (MQ=255)
cGTTCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAAt > 2:520355/1‑81 (MQ=255)
ttCGCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATcc > 2:520362/1‑81 (MQ=255)
gCCTGACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCaa > 1:520380/1‑81 (MQ=255)
gACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTg > 2:520375/1‑81 (MQ=255)
gACCATGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTg > 2:520368/1‑81 (MQ=255)
aTGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATc < 2:520317/81‑1 (MQ=255)
tGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa > 2:520373/1‑81 (MQ=255)
tGCAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCa > 2:520386/1‑81 (MQ=255)
cAACACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCAt < 2:520318/80‑1 (MQ=255)
aaCACGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGc < 1:520319/81‑1 (MQ=255)
acGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg < 2:520321/81‑1 (MQ=255)
acGCGCAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAg < 1:520320/81‑1 (MQ=255)
cgcAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGc > 2:520383/1‑80 (MQ=255)
cgcAGCAGCACGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCt < 2:520322/81‑1 (MQ=255)
cagcagcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTAc < 1:520323/81‑1 (MQ=255)
cagcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCa < 1:520324/81‑1 (MQ=255)
gcaCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAc > 1:520371/1‑81 (MQ=255)
caCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAcc > 2:520393/1‑81 (MQ=255)
aCGACTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAAccc > 2:520374/1‑81 (MQ=255)
|
CGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACGGCATGGGAAGGTTACACCAAAATCCCAACCTGGATCATGCAAGGCTACGCAACCC > NC_000913/3007980‑3008134
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
