Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,008,343 |
T→C |
V278V (GTT→GTC) |
ygeX → |
2,3‑diaminopropionate ammonia‑lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,008,343 | 0 | T | C | 100.0%
| 142.9
/ NA
| 40 | V278V (GTT→GTC) | ygeX | 2,3‑diaminopropionate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/19); total (21/19) |
GCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTA > NC_000913/3008268‑3008415
|
gCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTcggcgg > 1:520436/1‑81 (MQ=255)
cacaGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCg < 1:520381/79‑1 (MQ=255)
cacaGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGt > 2:520443/1‑81 (MQ=255)
cATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGatat < 2:520382/81‑1 (MQ=255)
cATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGatat > 1:520448/1‑81 (MQ=255)
ttattGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATgg < 1:520383/81‑1 (MQ=255)
tattGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGc > 1:520440/1‑81 (MQ=255)
attGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGc > 2:520428/1‑80 (MQ=255)
ttgttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGcc < 2:520385/80‑1 (MQ=255)
ttgttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGcc < 1:520384/80‑1 (MQ=255)
tgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcat > 2:520417/1‑81 (MQ=255)
gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcat < 1:520386/80‑1 (MQ=255)
aaCCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcatca > 2:520437/1‑81 (MQ=255)
ccTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATg < 1:520387/81‑1 (MQ=255)
gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATgg > 2:520447/1‑79 (MQ=255)
gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATgg > 2:520429/1‑79 (MQ=255)
cAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAgg > 1:520433/1‑81 (MQ=255)
aaaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggc > 1:520445/1‑81 (MQ=255)
aaaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggc > 2:520449/1‑81 (MQ=255)
aaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcc > 2:520430/1‑81 (MQ=255)
gCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctg < 1:520388/81‑1 (MQ=255)
cTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctgg > 2:520427/1‑81 (MQ=255)
cTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctgg > 1:520455/1‑81 (MQ=255)
gACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctggcc > 1:520441/1‑81 (MQ=255)
tcTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctggcct < 2:520389/80‑1 (MQ=255)
tttATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCg < 1:520390/81‑1 (MQ=255)
aTCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAAc > 1:520432/1‑81 (MQ=255)
cGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAAcc < 2:520391/80‑1 (MQ=255)
ggCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCg > 2:520451/1‑81 (MQ=255)
gCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgc < 2:520392/81‑1 (MQ=255)
cGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgct > 1:520438/1‑81 (MQ=255)
gTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctg < 1:520393/81‑1 (MQ=255)
tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg < 2:520394/81‑1 (MQ=255)
tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg < 1:520397/81‑1 (MQ=255)
tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg < 1:520396/81‑1 (MQ=255)
tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg < 1:520395/81‑1 (MQ=255)
aaGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgggct > 1:520442/1‑81 (MQ=255)
ggCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgggctgg > 1:520454/1‑81 (MQ=255)
aTCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATc > 2:520444/1‑81 (MQ=255)
gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCt < 2:520398/80‑1 (MQ=255)
gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTa < 1:520399/81‑1 (MQ=255)
|
GCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTA > NC_000913/3008268‑3008415
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
