Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,017,496 |
A→T |
E479D (GAA→GAT) |
ygfK → |
putative oxidoreductase, Fe‑S subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,017,496 | 0 | A | T | 97.6%
| 143.9
/ ‑3.9
| 42 | E479D (GAA→GAT) | ygfK | putative oxidoreductase, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (11/30); total (11/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 |
ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572
|
aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc < 2:522107/81‑1 (MQ=255)
cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGc < 2:522108/79‑1 (MQ=255)
cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCt < 2:522109/80‑1 (MQ=255)
tACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTAt > 2:522160/1‑81 (MQ=255)
gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGcc < 1:522110/81‑1 (MQ=255)
ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 1:522111/81‑1 (MQ=255)
ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 2:522112/81‑1 (MQ=255)
cccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc < 1:522113/80‑1 (MQ=255)
ccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAcgc < 1:522114/81‑1 (MQ=255)
cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 1:522115/81‑1 (MQ=255)
cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 1:522116/81‑1 (MQ=255)
tgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTgg < 2:522118/80‑1 (MQ=255)
tgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTgg < 1:522117/80‑1 (MQ=255)
gtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGaa > 2:522165/1‑81 (MQ=255)
ctgcctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTc < 2:522119/80‑1 (MQ=255)
gcgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTAc > 2:522168/1‑80 (MQ=255)
cgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTAcc < 1:522120/80‑1 (MQ=255)
cgcTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACca < 1:522121/81‑1 (MQ=255)
aTCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacg > 1:522161/1‑80 (MQ=255)
agcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGACGACGCGCTGGAACTCATCTACcaacgcaacg < 2:522122/81‑1 (MQ=255)
gcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCaacgcaacgc > 2:522172/1‑81 (MQ=255)
gcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCaacgcaacgc > 2:522171/1‑81 (MQ=255)
gATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTg > 1:522169/1‑81 (MQ=255)
atatTCAGGAATAGATCCGTCTGATTGGCGATAAGAGCTATGACGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGc < 1:522123/81‑1 (MQ=255)
ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt > 1:522183/1‑81 (MQ=255)
ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt > 2:522177/1‑81 (MQ=255)
ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt < 1:522124/81‑1 (MQ=255)
ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt < 1:522125/81‑1 (MQ=255)
aaTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTa > 1:522174/1‑81 (MQ=255)
aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc < 1:522126/81‑1 (MQ=255)
tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc < 2:522127/81‑1 (MQ=255)
tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc < 2:522128/81‑1 (MQ=255)
cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCg < 2:522129/80‑1 (MQ=255)
aTCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGt < 2:522130/81‑1 (MQ=255)
tCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTc < 1:522131/81‑1 (MQ=255)
cGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCat < 2:522132/81‑1 (MQ=255)
cGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCat < 1:522133/81‑1 (MQ=255)
gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg > 2:522181/1‑81 (MQ=255)
cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt < 1:522134/79‑1 (MQ=255)
cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGc < 1:522135/81‑1 (MQ=255)
ggCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCg < 2:522136/79‑1 (MQ=255)
gCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATc < 2:522137/81‑1 (MQ=255)
|
ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
