Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,017,496 |
A→T |
E479D (GAA→GAT) |
ygfK → |
putative oxidoreductase, Fe‑S subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,017,496 | 0 | A | T | 100.0%
| 143.9
/ NA
| 40 | E479D (GAA→GAT) | ygfK | putative oxidoreductase, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/23); total (17/23) |
ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572
|
aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc < 1:559852/81‑1 (MQ=255)
aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc < 2:559851/81‑1 (MQ=255)
cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTa > 2:559903/1‑81 (MQ=255)
gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCTTAATACATCCGTCTGCTTGGCGATCACCGCTATGcc > 1:559914/1‑81 (MQ=255)
gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGcc < 1:559853/81‑1 (MQ=255)
ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 2:559855/81‑1 (MQ=255)
ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg < 1:559854/81‑1 (MQ=255)
tGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGa > 2:559909/1‑81 (MQ=255)
gCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGa < 1:559856/80‑1 (MQ=255)
gCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc > 2:559912/1‑81 (MQ=255)
cccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc < 1:559857/80‑1 (MQ=255)
cccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAcgcg > 1:559900/1‑81 (MQ=255)
cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 1:559858/81‑1 (MQ=255)
cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt < 2:559859/81‑1 (MQ=255)
gtgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTg > 2:559920/1‑80 (MQ=255)
ttACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAAc < 1:559860/81‑1 (MQ=255)
aCTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACt > 2:559916/1‑80 (MQ=255)
ctgcctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTc < 2:559861/80‑1 (MQ=255)
cctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCAt < 1:559862/79‑1 (MQ=255)
tCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgc < 2:559863/80‑1 (MQ=255)
tCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacg < 2:559864/79‑1 (MQ=255)
aagcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgcaa < 2:559865/80‑1 (MQ=255)
aagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGctc < 1:559866/81‑1 (MQ=255)
agATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGctct < 2:559867/81‑1 (MQ=255)
ccGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGc < 2:559868/80‑1 (MQ=255)
ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt > 1:559924/1‑81 (MQ=255)
aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc > 2:559927/1‑81 (MQ=255)
aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc > 2:559919/1‑81 (MQ=255)
tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc < 1:559869/81‑1 (MQ=255)
aCATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCg > 2:559953/1‑81 (MQ=255)
cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCgg > 1:559918/1‑81 (MQ=255)
cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCgg > 1:559915/1‑81 (MQ=255)
tCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTc < 1:559870/81‑1 (MQ=255)
ccGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCa > 2:559917/1‑81 (MQ=255)
gTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCata > 1:559911/1‑81 (MQ=255)
gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg > 2:559922/1‑81 (MQ=255)
gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg > 1:559913/1‑81 (MQ=255)
cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt < 1:559871/79‑1 (MQ=255)
cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt < 2:559872/79‑1 (MQ=255)
gCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATc < 2:559873/81‑1 (MQ=255)
|
ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC > NC_000913/3017420‑3017572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
