Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	524	31.4	1748232	97.8%	1709770	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,867	G→A	G725G (GGC→GGT)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,867	0	G	A	100.0%	114.3 / NA	34	G725G (GGC→GGT)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/21); total (13/21)

CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943

cgcgCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACAcc                                                                            >  1:528199/1‑81 (MQ=255)

 gcgcCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCg                                                                           <  2:528137/81‑1 (MQ=255)

    cccTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATc                                                                        <  1:528139/81‑1 (MQ=255)

    cccTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATc                                                                        <  2:528138/81‑1 (MQ=255)

        gACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTc                                                                    >  2:528140/1‑81 (MQ=255)

         aCGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTc                                                                    <  2:528141/80‑1 (MQ=255)

           gggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCa                                                                 >  2:528180/1‑81 (MQ=255)

           gggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCa                                                                 >  1:528182/1‑81 (MQ=255)

            ggTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCAt                                                                <  2:528142/81‑1 (MQ=255)

              ttAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAc                                                              <  2:528143/81‑1 (MQ=255)

               tAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAcc                                                             >  2:528185/1‑81 (MQ=255)

                aaCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAccc                                                            >  2:528190/1‑81 (MQ=255)

                aaCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAcc                                                             <  2:528144/80‑1 (MQ=255)

                 aCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAccc                                                            <  2:528145/80‑1 (MQ=255)

                 aCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCg                                                           >  2:528183/1‑81 (MQ=255)

                          cGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccacc                                                    <  2:528146/79‑1 (MQ=255)

                           gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 >  1:528208/1‑81 (MQ=255)

                           gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 <  2:528147/81‑1 (MQ=255)

                              ttGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              >  1:528186/1‑81 (MQ=255)

                               tGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              <  1:528148/80‑1 (MQ=255)

                                      cGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGaa                                      <  1:528149/81‑1 (MQ=255)

                                         tATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATg                                    >  1:528193/1‑80 (MQ=255)

                                           tGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAg                                 <  2:528150/81‑1 (MQ=255)

                                           tGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAg                                 <  2:528151/81‑1 (MQ=255)

                                             aCCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGa                                <  1:528152/80‑1 (MQ=255)

                                               ccGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAg                             >  2:528184/1‑81 (MQ=255)

                                                  gTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGttt                          <  1:528153/81‑1 (MQ=255)

                                                     cAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTAt                       <  2:528154/81‑1 (MQ=255)

                                                           gTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGt                 <  2:528155/81‑1 (MQ=255)

                                                            tGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGtt                <  2:528156/81‑1 (MQ=255)

                                                            tGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGt                 >  1:528195/1‑80 (MQ=255)

                                                                aaaTGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAgg            <  1:528157/81‑1 (MQ=255)

                                                                      gcTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGaa      <  1:528158/81‑1 (MQ=255)

                                                                       cTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGaaa     >  1:528201/1‑81 (MQ=255)

                                                                           cacaCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGt  <  2:528159/80‑1 (MQ=255)

CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A