Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,073,292 |
C→A |
V134F (GTT→TTT) |
epd ← |
D‑erythrose‑4‑phosphate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,073,292 | 0 | C | A | 100.0%
| 126.2
/ NA
| 36 | V134F (GTT→TTT) | epd | D‑erythrose‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/17); total (19/17) |
CAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAT > NC_000913/3073216‑3073369
|
cAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTc < 1:533609/81‑1 (MQ=255)
aaTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcg < 2:533611/81‑1 (MQ=255)
aaTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCC‑CACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgc < 2:533610/81‑1 (MQ=255)
aTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgc > 1:533659/1‑81 (MQ=255)
ttCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgcg < 1:533612/81‑1 (MQ=255)
cGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGt < 2:533613/80‑1 (MQ=255)
gTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTc > 1:533656/1‑80 (MQ=255)
gTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCgag < 1:533614/80‑1 (MQ=255)
aaGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTAc > 2:533678/1‑81 (MQ=255)
gTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGcc < 2:533615/81‑1 (MQ=255)
aGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAg > 1:533667/1‑80 (MQ=255)
aGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAg > 2:533662/1‑80 (MQ=255)
aaaCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAt > 1:533647/1‑81 (MQ=255)
cGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtgtg < 2:533616/81‑1 (MQ=255)
gATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtgtg > 2:533658/1‑80 (MQ=255)
gATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtgtg > 1:533673/1‑80 (MQ=255)
aTGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGaa < 1:533617/81‑1 (MQ=255)
cGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGaa > 2:533665/1‑78 (MQ=255)
gtgtTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAagag < 1:533618/81‑1 (MQ=255)
ccGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGAGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtt > 2:533645/1‑81 (MQ=255)
cGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACttt < 1:533619/81‑1 (MQ=255)
aCGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtttttt > 1:533668/1‑81 (MQ=255)
gAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTgg > 1:533681/1‑81 (MQ=255)
aaGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGc > 2:533674/1‑81 (MQ=255)
tCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGCTTTTAAAAGTTCACTTTTTTTGCCCcggcg > 1:864502/1‑81 (MQ=255)
tCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcggcg > 2:533663/1‑81 (MQ=255)
cTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCggcggc < 2:533620/81‑1 (MQ=255)
ttGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAata < 2:533621/81‑1 (MQ=255)
aCGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgc < 1:533622/81‑1 (MQ=255)
cGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgcg < 1:533623/81‑1 (MQ=255)
gCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgcgc > 2:533672/1‑81 (MQ=255)
gCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGAACTTTTTTGGCCCCGGCGGCAATATgcgc > 1:533669/1‑81 (MQ=255)
cGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCtt < 2:533625/81‑1 (MQ=255)
cGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCtt < 2:533624/81‑1 (MQ=255)
cGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCtt > 1:533684/1‑81 (MQ=255)
cGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCtt > 2:533685/1‑81 (MQ=255)
aacaaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGGGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGc < 2:533626/80‑1 (MQ=255)
aacaaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGc < 1:533627/80‑1 (MQ=255)
caaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAt < 2:533628/81‑1 (MQ=255)
caaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAt > 2:533660/1‑81 (MQ=255)
caaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAt < 1:533629/81‑1 (MQ=255)
|
CAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAT > NC_000913/3073216‑3073369
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
